Product Name
TLR3, Recombinant Protein
Popular Item
Full Product Name
TLR3 protein
Product Synonym Names
TLR3; CD283 antigen protein; TLR-3 protein; TLR-3; Toll-like receptor 3; TLR 3 protein; TLR 3
Product Gene Name
TLR3 recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
Region of TLR3 protein correspond ing to amino acids STTKCTVSHE VADCSHLKLT QVPDDLPTNI TVLNLTHNQL RRLPAANFTR YSQLTSLDVG FNTISKLEPE LCQKLPMLKV LNLQHNELSQ LSDKTFAFCT NLTELHLMSN SIQKIKNNPF VKQKNLITLD LSHNGLSSTK LGTQVQLENL QELLLSNNKI QALKSEELDI FANSSLKKLE LSSNQIKEFS PGCFHAIGRL FGLFLNNVQL GPSLTEKLCL ELANTSIRNL SLSNSQLSTT SNTTFLGLKW TNLTMLDLSY NNLNVVGNDS FAWLPQLEYF FLEYNNIQHL FSHSLHGLFN VRYLNLKRSF TKQSISLASL PKIDDFSFQW LKCLEHLNME DNDIPGIKSN MFTGLINLKY LSLSNSFTSL RTLTNETFVS LAHSPLHILN LTKNKISKIE SDAFSWLGHL EVLDLGLNEI GQELTGQEWR GLENIFEIYL SYNKYLQLTR NSFALVPSLQ RLMLRRVALK NVDSSPSPFQ PLRNLTILDL SNNNIANIND DMLEGLEKLE ILDLQHNNLA RLWKHANPGG PIYFLKGLSH LHILNLESNG FDEIPVEVFK DLFELKIIDL GLNNLNTLPA SVFNNQVSLK SLNLQKNLIT SVEKKVFGPA FRNLTELDMR FNPFDCTCES IAWFVNWINE THTNIPELSS HYLCNTPPHY HGFPVRLFDT SSCKDSAPFE L.
3D Structure
ModBase 3D Structure for Q7TNI8
Purity/Purification
>95% by SDS-PAGE gel and HPLC analysis
Form/Format
Lyophilized from 20mM Sodium Phosphate, pH 7.5. Centrifuge prior to opening. Reconstitute in water to 0.1-1.0mg/ml. Do not vortex.
Concentration
25ug, lyophilized (lot specific)
Biological Significance
TLR-3 is a single-pass type I receptor that binds to and signals the presence of microbial pathogens and double stranded RNA (dsRNA) viruses. Signaling through TLR-3 can promote the NF-?B pathway to initiate innate and adaptive immune responses to bacterial and viral infections, as well as the p53 pathway to trigger apoptosis in cells infected with dsRNA viruses. TLR-3 belongs to a family of structurally-related toll-like receptors (TLRs) containing an N-terminal domain rich in leucine repeats and a C-terminal intracellular Toll/interleukin (IL)-1 (TIL) domain. TLR-3 is expressed primarily in dendritic cells of the placenta and pancreas where it can reside on both sides of the plasma membrane and in the endosomal compartment of the cells.
Bioactivity
Determined by its ability to inhibit IL-8 secretion induced by Poly I:C in the TLR3 receptor-expressing HEK293 cells (HEK293 TLR3r). The ED50for this effect is approximately 30 ug/ml in the presence of 100 ug/ml of Poly I:C.
Contaminants
Endotoxin level is <0.1ng/ug of protein
Biohazard
Use standard laboratory precautions when handling this material
Preparation and Storage
Ships ambient or refrigerated. Upon receipt store at 4 degree C short term, -20 degree C long term. Avoid repeat freeze-thaws.
Other Notes
Small volumes of TLR3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TLR3 recombinant protein
Purified recombinant Human TLR3 protein
Product Categories/Family for TLR3 recombinant protein
Cytokines & Growth Factors
Applications Tested/Suitable for TLR3 recombinant protein
User optimized
NCBI/Uniprot data below describe general gene information for TLR3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_942086.1
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NCBI GenBank Nucleotide #
NM_198791.1
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UniProt Primary Accession #
Q7TNI8
[Other Products]
NCBI Official Full Name
toll-like receptor 3
NCBI Official Synonym Full Names
toll-like receptor 3
NCBI Official Symbol
Tlr3??[Similar Products]
NCBI Protein Information
toll-like receptor 3
UniProt Protein Name
Toll-like receptor 3
UniProt Synonym Protein Names
Toll-like receptor 3
Protein Family
Toll-like receptor
UniProt Gene Name
Tlr3??[Similar Products]
UniProt Entry Name
Q7TNI8_RAT
NCBI Summary for TLR3
human homolog induces activation of NF-kappaB; mouse homolog plays a role in the production of inflammatory cytokines [RGD, Feb 2006]
UniProt Comments for TLR3
TLR3: a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. Most abundantly expressed in placenta and pancreas. Restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection. Acts via MyD88 and TRAF6, leading to the activation of NF-kappaB and the production of type I interferon.
Protein type: Receptor, cytokine; Membrane protein, integral
Cellular Component: cell surface; lysosomal membrane; cytoplasm; integral to membrane; intracellular
Molecular Function: identical protein binding; transmembrane receptor activity; double-stranded RNA binding; receptor activity
Biological Process: positive regulation of apoptosis; positive regulation of interleukin-12 production; microglial cell activation; positive regulation of JNK cascade; defense response; positive regulation of NF-kappaB import into nucleus; positive regulation of interferon-alpha biosynthetic process; toll-like receptor 3 signaling pathway; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; response to exogenous dsRNA; positive regulation of interferon-beta production; positive regulation of interferon type I production; defense response to virus; positive regulation of I-kappaB kinase/NF-kappaB cascade; response to virus; MyD88-independent toll-like receptor signaling pathway; male gonad development; response to dsRNA; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of chemokine production; positive regulation of toll-like receptor signaling pathway; positive regulation of chemokine biosynthetic process; positive regulation of interferon-beta biosynthetic process; toll-like receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; I-kappaB phosphorylation; positive regulation of inflammatory response; microglial cell activation during immune response; positive regulation of interferon-gamma biosynthetic process
Research Articles on TLR3
1. IL-1beta is the key trigger of neuroinflammation and that IL-1ra prevents the neuroinflammation entering the chronic state
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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