Product Name
POLR1C, siRNA
Full Product Name
POLR1C siRNA (Human)
Product Synonym Names
POLR1E; DNA-directed RNA polymerases I and III subunit RPAC1; DNA-directed RNA polymerase I subunit C; RNA polymerases I and III subunit AC1; AC40; DNA-directed RNA polymerases I and III 40 kDa polypeptide; RPA40; RPA39; RPC40
Product Gene Name
POLR1C sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O15160
Specificity
POLR1C siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human POLR1C gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of POLR1C sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
POLR1C sirna
siRNA to inhibit POLR1C expression using RNA interference
Applications Tested/Suitable for POLR1C sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for POLR1C. It may not necessarily be applicable to this product.
NCBI Accession #
NP_976035.1
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NCBI GenBank Nucleotide #
NM_203290.2
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UniProt Primary Accession #
O15160
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UniProt Secondary Accession #
O75395; Q5JTE3[Other Products]
UniProt Related Accession #
O15160[Other Products]
Molecular Weight
38,647 Da
NCBI Official Full Name
DNA-directed RNA polymerases I and III subunit RPAC1
NCBI Official Synonym Full Names
polymerase (RNA) I polypeptide C, 30kDa
NCBI Official Symbol
POLR1C??[Similar Products]
NCBI Official Synonym Symbols
RPA5; TCS3; RPA39; RPA40; RPAC1
??[Similar Products]
NCBI Protein Information
DNA-directed RNA polymerases I and III subunit RPAC1
UniProt Protein Name
DNA-directed RNA polymerases I and III subunit RPAC1
UniProt Synonym Protein Names
AC40; DNA-directed RNA polymerases I and III 40 kDa polypeptide; RPA40; RPA39; RPC40
Protein Family
DNA-directed RNA polymerases
UniProt Gene Name
POLR1C??[Similar Products]
UniProt Synonym Gene Names
POLR1E; DNA-directed RNA polymerase I subunit C; RNA polymerases I and III subunit AC1; RPA40??[Similar Products]
UniProt Entry Name
RPAC1_HUMAN
NCBI Summary for POLR1C
The protein encoded by this gene is a subunit of both RNA polymerase I and RNA polymerase III complexes. The encoded protein is part of the Pol core element. Defects in this gene have been associated with Treacher Collins syndrome (TCS). [provided by RefSeq, Mar 2011]
UniProt Comments for POLR1C
RPA40: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleft and probably a clamp element that moves to open and close the cleft. Defects in POLR1C are the cause of Treacher Collins syndrome type 3 (TCS3). A form of Treacher Collins syndrome, a disorder of craniofacial development. Treacher Collins syndrome is characterized by a combination of bilateral downward slanting of the palpebral fissures, colobomas of the lower eyelids with a paucity of eyelashes medial to the defect, hypoplasia of the facial bones, cleft palate, malformation of the external ears, atresia of the external auditory canals, and bilateral conductive hearing loss. Belongs to the archaeal RpoD/eukaryotic RPB3 RNA polymerase subunit family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.7.7.6; Transcription initiation complex; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Transferase
Chromosomal Location of Human Ortholog: 6p21.1
Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; cytosol; DNA-directed RNA polymerase I complex
Molecular Function: protein dimerization activity; DNA binding; DNA-directed RNA polymerase activity
Biological Process: transcription from RNA polymerase III promoter; termination of RNA polymerase III transcription; negative regulation of gene expression, epigenetic; positive regulation of interferon type I production; transcription from RNA polymerase I promoter; RNA elongation from RNA polymerase I promoter; innate immune response; gene expression; transcription initiation from RNA polymerase I promoter; termination of RNA polymerase I transcription; regulation of gene expression, epigenetic; RNA elongation from RNA polymerase III promoter
Disease: Leukodystrophy, Hypomyelinating, 11; Treacher Collins Syndrome 3
Research Articles on POLR1C
1. Mutations in TCOF1, POLR1C and POLR1D have all been implicated in causing TCS
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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