For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Small volumes of anti-HYAL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Polyclonal

Western Blot (WB), Immunohistochemistry (IHC)

Western blot analysis of extracts of various cell lines, using HYAL1 Antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human liver injury using HYAL1 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using HYAL1 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat kidney using HYAL1 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using HYAL1 antibody at dilution of 1:100 (40x lens).

435

hyaluronoglucosaminidase 1

hyaluronidase-1; luCa-1; plasma hyaluronidase; tumor suppressor LUCA-1; lung carcinoma protein 1

Hyaluronidase-1

LUCA1; Hyal-1; LuCa-1??[Similar Products]

HYAL1_HUMAN

This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

HYAL1: May have a role in promoting tumor progression. May block the TGFB1-enhanced cell growth. Defects in HYAL1 are the cause of mucopolysaccharidosis type 9 (MPS9); also called hyaluronidase deficiency. MPS9 is a lysosomal storage disease characterized by high hyaluronan (HA) concentration in the serum. The clinical features are periarticular soft tissue masses, mild short stature and acetabular erosions, and absence of neurological or visceral involvement. Belongs to the glycosyl hydrolase 56 family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; EC 3.2.1.35; Glycan Metabolism - glycosaminoglycan degradation; Secreted; Hydrolase

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: lysosomal lumen; extracellular space; lysosome; cytoplasm; cytoplasmic vesicle

Molecular Function: viral receptor activity; hyaluronan synthase activity; transcription factor binding; hyalurononglucosaminidase activity

Biological Process: positive regulation of cell adhesion; glycosaminoglycan metabolic process; response to virus; pathogenesis; positive regulation of cell growth; hyaluronan catabolic process; chondroitin sulfate metabolic process; response to antibiotic; positive regulation of angiogenesis; response to reactive oxygen species; hyaluronan biosynthetic process; cartilage development; carbohydrate metabolic process; chondroitin sulfate catabolic process; negative regulation of cell growth; inflammatory response; positive regulation of growth; hyaluronan metabolic process; positive regulation of epithelial cell proliferation

Disease: Mucopolysaccharidosis, Type Ix

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