Product Name
HYAL1, siRNA
Full Product Name
HYAL1 siRNA (Mouse)
Product Synonym Names
Hyaluronidase-1; Hyal-1; Hyaluronoglucosaminidase-1
Product Gene Name
HYAL1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q91ZJ9
Specificity
HYAL1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse HYAL1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of HYAL1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
HYAL1 sirna
siRNA to inhibit HYAL1 expression using RNA interference
Applications Tested/Suitable for HYAL1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for HYAL1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_032343.2
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NCBI GenBank Nucleotide #
NM_008317.4
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UniProt Primary Accession #
Q91ZJ9
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UniProt Secondary Accession #
O70229; Q8CE62; Q8QZX3; Q8VBW7; Q8VDK0; B1AV90[Other Products]
UniProt Related Accession #
Q91ZJ9[Other Products]
Molecular Weight
51,436 Da
NCBI Official Full Name
hyaluronidase-1
NCBI Official Synonym Full Names
hyaluronoglucosaminidase 1
NCBI Official Symbol
Hyal1??[Similar Products]
NCBI Official Synonym Symbols
Hya1; Hyal-1
??[Similar Products]
NCBI Protein Information
hyaluronidase-1
UniProt Protein Name
Hyaluronidase-1
UniProt Synonym Protein Names
Hyaluronoglucosaminidase-1
UniProt Gene Name
Hyal1??[Similar Products]
UniProt Synonym Gene Names
Hyal-1??[Similar Products]
UniProt Entry Name
HYAL1_MOUSE
UniProt Comments for HYAL1
HYAL1: May have a role in promoting tumor progression. May block the TGFB1-enhanced cell growth. Defects in HYAL1 are the cause of mucopolysaccharidosis type 9 (MPS9); also called hyaluronidase deficiency. MPS9 is a lysosomal storage disease characterized by high hyaluronan (HA) concentration in the serum. The clinical features are periarticular soft tissue masses, mild short stature and acetabular erosions, and absence of neurological or visceral involvement. Belongs to the glycosyl hydrolase 56 family. 7 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Hydrolase; EC 3.2.1.35; Glycan Metabolism - glycosaminoglycan degradation; Secreted
Cellular Component: extracellular space; lysosome; cytoplasm; extracellular region; cytoplasmic vesicle
Molecular Function: viral receptor activity; hydrolase activity; hydrolase activity, acting on glycosyl bonds; hyaluronan synthase activity; catalytic activity; transcription factor binding; hyalurononglucosaminidase activity
Biological Process: positive regulation of cell adhesion; metabolic process; response to virus; positive regulation of cell growth; hyaluronan catabolic process; response to antibiotic; response to reactive oxygen species; positive regulation of angiogenesis; hyaluronan biosynthetic process; carbohydrate metabolic process; negative regulation of cell growth; inflammatory response; hyaluronan metabolic process; positive regulation of growth; positive regulation of epithelial cell proliferation
Research Articles on HYAL1
1. The activity of the processed form of Hyal-1 was largely underestimated.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
