For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

Mouse IgG1, Kappa

ABM15D5

Mouse

Purified (Liquid)

0.5 mg/ml, 200 μl (lot specific)

Store antibody at 4 degree C, stable for 6 months. For long-term storage, store at -20 degree C. Avoid repeated freeze and thaw cycles. Expire 1 year from date of purchase.

Small volumes of anti-TLR3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

TLR

Western Blot (WB), Flow Cytometry (FC/FACS)

Expression analysis of TLR3. Anti- TLR3 antibody (Clone: ABM15D5) was used at 2 μg/ml on Ovary lysate.

Intracellular flow analysis of TLR3 in human PBMC (monocytes) using 2.0 μg/10^6 cells of TLR3antibody (Clone: ABM15D5). Green represents isotype control; red represents anti-TLR3 antibody. Goat anti-mouse PE conjugate was used as secondary.

Intracellular flow analysis of TLR3 in human PBMC (lymphocytes) using 2.0 μg/10^6 cells of TLR3antibody (Clone: ABM15D5). Green represents isotype control; red represents anti-TLR3 antibody. Goat anti-mouse PE conjugate was used as secondary.

72,937 Da

toll-like receptor 3

toll-like receptor 3

Toll-like receptor 3

TLR3_HUMAN

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. It may thus play a role in host defense against viruses. Use of alternative polyadenylation sites to generate different length transcripts has been noted for this gene. [provided by RefSeq, Jul 2008]

TLR3: a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. Most abundantly expressed in placenta and pancreas. Restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection. Acts via MyD88 and TRAF6, leading to the activation of NF-kappaB and the production of type I interferon.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 4q35

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; cell surface; membrane; lysosomal membrane; integral to plasma membrane; cytoplasm; endosome membrane

Molecular Function: identical protein binding; protein binding; transmembrane receptor activity; double-stranded RNA binding; receptor activity

Biological Process: negative regulation of osteoclast differentiation; I-kappaB kinase/NF-kappaB cascade; positive regulation of apoptosis; positive regulation of interleukin-12 production; positive regulation of JNK cascade; positive regulation of interferon-alpha biosynthetic process; positive regulation of NF-kappaB import into nucleus; toll-like receptor 3 signaling pathway; signal transduction; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; response to exogenous dsRNA; positive regulation of interferon-beta production; inflammatory response; toll-like receptor 4 signaling pathway; defense response to virus; MyD88-independent toll-like receptor signaling pathway; male gonad development; activation of NF-kappaB-inducing kinase; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of toll-like receptor signaling pathway; positive regulation of chemokine production; hyperosmotic response; positive regulation of chemokine biosynthetic process; detection of virus; defense response to bacterium; positive regulation of interferon-beta biosynthetic process; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; I-kappaB phosphorylation; positive regulation of interferon-gamma biosynthetic process; microglial cell activation during immune response; positive regulation of inflammatory response

Disease: Human Immunodeficiency Virus Type 1, Susceptibility To

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.