TLR3; CD283; IIAE2; Toll-like receptor 3; toll-like receptor 3

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

TLR3 Antibody is affinity chromatography purified via peptide column.

Liquid

1 mg/mL (lot specific)

TLR3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Small volumes of anti-TLR3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

TLR3 Antibody: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors so the organism can respond to potential infection. TLR3 is known to recognize viral double-stranded (ds) RNA, a molecular pattern associated with viral infection. Recently it has been shown to recognize viruses such as Influenza A and West Nile Virus and can mediate entry of at least West Nile Virus.

ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

TLR3 antibody can be used for detection of TLR3 by Western blot at 0.5 - 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 2 mug/mL.

Western blot analysis of TLR3 in EL4 cell lysate with TLR3 antibody at 0.5 μg/mL.

Immunocytochemistry of TLR3 in K562 cells with TLR3 antibody at 10 μg/mL.

Immunofluorescence of TLR3 in K562 cells with TLR3 antibody at 2 μg/mL.

Predicted: 97 kDa

Observed: 93 kDa

toll-like receptor 3

toll-like receptor 3

Toll-like receptor 3

TLR3_HUMAN

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. It may thus play a role in host defense against viruses. Use of alternative polyadenylation sites to generate different length transcripts has been noted for this gene. [provided by RefSeq, Jul 2008]

TLR3: a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. Most abundantly expressed in placenta and pancreas. Restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection. Acts via MyD88 and TRAF6, leading to the activation of NF-kappaB and the production of type I interferon.

Protein type: Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q35

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; cell surface; membrane; lysosomal membrane; integral to plasma membrane; cytoplasm; endosome membrane

Molecular Function: identical protein binding; protein binding; transmembrane receptor activity; double-stranded RNA binding; receptor activity

Biological Process: I-kappaB kinase/NF-kappaB cascade; negative regulation of osteoclast differentiation; positive regulation of interleukin-12 production; positive regulation of apoptosis; positive regulation of JNK cascade; positive regulation of NF-kappaB import into nucleus; positive regulation of interferon-alpha biosynthetic process; toll-like receptor 3 signaling pathway; signal transduction; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; response to exogenous dsRNA; positive regulation of interferon-beta production; inflammatory response; toll-like receptor 4 signaling pathway; defense response to virus; MyD88-independent toll-like receptor signaling pathway; male gonad development; activation of NF-kappaB-inducing kinase; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of toll-like receptor signaling pathway; positive regulation of chemokine production; hyperosmotic response; positive regulation of chemokine biosynthetic process; detection of virus; defense response to bacterium; positive regulation of interferon-beta biosynthetic process; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; I-kappaB phosphorylation; positive regulation of interferon-gamma biosynthetic process; microglial cell activation during immune response; positive regulation of inflammatory response

Disease: Human Immunodeficiency Virus Type 1, Susceptibility To

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