Full Product Name
Anti-DARS2 Antibody
Product Synonym Names
Aspartate--tRNA ligase, mitochondrial; Aspartyl-tRNA synthetase; AspRS; DARS2; aspartyl-tRNA synthetase 2, mitochondrial
Product Gene Name
anti-DARS2 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q6PI48
Species Reactivity
Human
No cross reactivity with other proteins.
Purity/Purification
Immunogen Affinity Purified
Immunogen
E Coli-derived human DARS2 recombinant protein (Position: D334-A448).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500mug/ml.
Relevant Detection Systems
We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit for IHC(P).
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, store at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-DARS2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-DARS2 antibody
Description: Rabbit IgG polyclonal antibody for DARS2 detection. Tested with WB, IHC-P, Direct ELISA in Human.
Background: DARS2 contains conserved residues involved in ATP binding, tRNA binding, and aspartic acid recognition, as well as catalytic site motifs characteristic of amino acid tRNA synthetases. The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL). The International Radiation Hybrid Mapping Consortium mapped the DARS2 gene to chromosome 1.
Applications Tested/Suitable for anti-DARS2 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Direct ELISA (EIA)
Application Notes for anti-DARS2 antibody
WB: 0.1-0.5ug/ml
IHC-P: 0.5-1ug/ml
Direct ELISA: 0.1-0.5ug/ml
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested.
NCBI/Uniprot data below describe general gene information for DARS2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_060592.2
[Other Products]
NCBI GenBank Nucleotide #
NM_018122.5
[Other Products]
UniProt Primary Accession #
Q6PI48
[Other Products]
UniProt Related Accession #
Q6PI48[Other Products]
Molecular Weight
73,563 Da
NCBI Official Full Name
aspartate--tRNA ligase, mitochondrial isoform 1
NCBI Official Synonym Full Names
aspartyl-tRNA synthetase 2, mitochondrial
NCBI Official Symbol
DARS2??[Similar Products]
NCBI Official Synonym Symbols
LBSL; ASPRS; MT-ASPRS
??[Similar Products]
NCBI Protein Information
aspartate--tRNA ligase, mitochondrial
UniProt Protein Name
Aspartate--tRNA ligase, mitochondrial
UniProt Synonym Protein Names
Aspartyl-tRNA synthetase; AspRS
UniProt Gene Name
DARS2??[Similar Products]
UniProt Synonym Gene Names
AspRS??[Similar Products]
NCBI Summary for DARS2
The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL). [provided by RefSeq, Nov 2009]
Product References and Citations for anti-DARS2 antibody
1. Isohanni, P., Linnankivi, T., Buzkova, J., Lonnqvist, T., Pihko, H., Valanne, L., Tienari, P. J., Elovaara, I., Pirttila, T., Reunanen, M., Koivisto, K., Marjavaara, S., Suomalainen, A. DARS2 mutations in mitochondrial leucoencephalopathy and multiple sclerosis. J. Med. Genet. 47: 66-70, 2010. 2. Miyake, N., Yamashita, S., Kurosawa, K., Miyatake, S., Tsurusaki, Y., Doi, H., Saitsu, H., Matsumoto, N. A novel homozygous mutation of DARS2 may cause a severe LBSL variant. (Letter) Clin. Genet. 80: 293-296, 2011.
Research Articles on DARS2
1. Results show that DARS2 is strongly upregulated in hepatocellular carcinoma (HCC) and is associated with HCC progression. DARS2 promotes HCC tumorigenesis by accelerating cell cycle progression and attenuating cell apoptosis.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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