For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of CDK5RAP2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7231853 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CDK5 regulatory subunit associated protein 2 (CDK5RAP2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CDK5RAP2. The ELISA analytical biochemical technique of the MBS7231853 kit is based on CDK5RAP2 antibody-CDK5RAP2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CDK5RAP2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CDK5RAP2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Cell Biology

206,323 Da

CDK5 regulatory subunit associated protein 2

CDK5 regulatory subunit-associated protein 2; centrosomin; centrosomal protein 215 kDa; CDK5 activator-binding protein C48

CDK5 regulatory subunit-associated protein 2

CEP215; KIAA1633??[Similar Products]

CK5P2_HUMAN

This gene encodes a regulator of CDK5 (cyclin-dependent kinase 5) activity. The protein encoded by this gene is localized to the centrosome and Golgi complex, interacts with CDK5R1 and pericentrin (PCNT), plays a role in centriole engagement and microtubule nucleation, and has been linked to primary microcephaly and Alzheimer's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]

CDK5RAP2: Potential regulator of CDK5 activity via its interaction with CDK5R1. Negative regulator of centriole disengagement (licensing) which maintains centriole engagement and cohesion. Involved in regulation of mitotic spindle orientation. Plays a role in the spindle checkpoint activation by acting as a transcriptional regulator of both BUBR1 and MAD2 promoter. Together with MAPRE1, it may promote microtubule polymerization, bundle formation, growth and dynamics at the plus ends. Defects in CDK5RAP2 are the cause of microcephaly primary type 3 (MCPH3). A disorder defined as a head circumference more than 3 standard deviations below the age- related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 9q33.2

Cellular Component: spindle pole; Golgi apparatus; centrosome; microtubule; pericentriolar material; cytoskeleton; perinuclear region of cytoplasm; cytoplasm; cytosol; cell junction

Molecular Function: tubulin binding; calmodulin binding; protein binding; microtubule binding; protein kinase binding

Biological Process: regulation of neuron differentiation; organelle organization and biogenesis; positive regulation of transcription, DNA-dependent; microtubule cytoskeleton organization and biogenesis; negative regulation of centriole replication; chromosome segregation; negative regulation of neuron differentiation; establishment of mitotic spindle orientation; neurogenesis; centrosome organization and biogenesis; brain development; mitotic cell cycle; G2/M transition of mitotic cell cycle; microtubule bundle formation

Disease: Microcephaly 3, Primary, Autosomal Recessive

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