Tlr3

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

Rat IgG2b, Kappa

ABM24E5

Rat

Protein G Chromatography

Purified (Liquid)

Store the antibody at 4 degree C, stable for 6 months. For long-term storage, store at -20 degree C. Avoid repeated freeze and thaw cycles.

Small volumes of anti-mTLR3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Mouse Toll-like receptor 3 (mTLR3) belongs to a family of evolutionary conserved innate immune recognition molecules and recognizes double-stranded RNA, a molecular pattern associated with viral infections. It has seven exons. mTlr3 show high homology to hTLR3 in their predicted cDNA and protein sequences. The intracellular region of mTlr3 has remarkable homology with the same region of others mTlrs. mTlr3 mRNA is ubiquitously expressed in tissues, being expressed highest in spleen, kidney and lung. Its activation by the synthetic ligand polyinosine:polycytidylic acid (poly I:C) or by mRNA rapidly causes growth cone collapse and irreversibly inhibits neurite extension independent of NF-kappaB. The high levels of mTlr3 expression in Mz B cells perhaps contribute to their ability to clear antigen from the blood.

TLR

Western Blot (WB), FACS, Immunohistochemistry (IHC)

Western blot analysis: 2-4 ug/ml; FACS analysis: 0.5 ug/10^6 cells; Immunohistochemical analysis: 10ug/ml

Western Blot analysis of mTLR3. Anti- mTLR3 antibody (Clone: ABM24E5) was used at 2 ug/ml on mouse brain lysate.

Intracellular flow analysis of mTLR3 in m Splenocytes using 0.5 tJg/1 0"6 cells of mTLR3 antibody (Clone: ABM24E5). Green represents isotype control; red represents anti-mTLR3 antibody. Goat anti-Rat FITe conjugate was used as secondary antibody .

Immunohistochemical analysis of mTLR3 in human Liver tissue using mTLR3 antibody (Clone:ABM24ES) at 10 ug/ml.

toll-like receptor 3

toll-like receptor 3

Toll-like receptor 3

TLR3_MOUSE

TLR3: a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. Most abundantly expressed in placenta and pancreas. Restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection. Acts via MyD88 and TRAF6, leading to the activation of NF-kappaB and the production of type I interferon.

Protein type: Receptor, cytokine; Membrane protein, integral

Cellular Component: cell surface; membrane; endoplasmic reticulum; lysosomal membrane; cytoplasm; integral to membrane; intracellular; endosome

Molecular Function: identical protein binding; protein binding; transmembrane receptor activity; RNA binding; double-stranded RNA binding; receptor activity

Biological Process: positive regulation of interleukin-12 production; positive regulation of JNK cascade; microglial cell activation; positive regulation of NF-kappaB import into nucleus; positive regulation of interferon-alpha biosynthetic process; defense response; toll-like receptor 3 signaling pathway; signal transduction; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; response to exogenous dsRNA; positive regulation of interferon-beta production; positive regulation of interferon type I production; inflammatory response; defense response to virus; positive regulation of cytokine production; positive regulation of I-kappaB kinase/NF-kappaB cascade; immune system process; response to virus; MyD88-independent toll-like receptor signaling pathway; response to dsRNA; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of chemokine production; positive regulation of toll-like receptor signaling pathway; positive regulation of chemokine biosynthetic process; positive regulation of interferon-beta biosynthetic process; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; I-kappaB phosphorylation; microglial cell activation during immune response; positive regulation of interferon-gamma biosynthetic process; positive regulation of inflammatory response

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.