Full Product Name
FZD4 Antibody: APC/Cy7
Product Synonym Names
Rabbit Anti-Human FZD4 Polyclonal; Frizzled-4 Antibody; CD 344 antigen Antibody; CD 344 Antibody; FZD4_HUMAN Antibody; EVR1 Antibody; FzE4 Antibody; FZD4 Antibody; Fz 4 Antibody; Frizzled family receptor 4 Antibody; WNT receptor frizzled 4 Antibody; CD344 antigen Antibody; MGC34390 Antibody; CD344 Antibody; FZD4S Antibody; Frizzled-4 Antibody; Frizzled (Drosophila) homolog 4 Antibody; CD344 Antibody; Fz4 Antibody; GPCR Antibody; Frizzled homolog 4 (Drosophila) Antibody; hFz4 Antibody; Frizzled 4 Antibody; FEVR Antibody; Fz-4 Antibody
Product Gene Name
anti-FZD4 antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9ULV1
Species Reactivity
Human, Mouse
Specificity
Detects 60 kDa.
Purity/Purification
Peptide Affinity Purified
Form/Format
PBS pH 7.4, 50% Glycerol, 0.09% Sodium Azide
Concentration
1 mg/ml (lot specific)
Immunogen
Synthetic peptide of Human FZD4 (100-200 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
Cellular Localization
Membrane; Multi-Pass Membrane Protein; Cell Membrane; Multi-Pass Membrane Protein
Tissue Specificity
Almost ubiquitous. Largely expressed in ***** heart, skeletal muscle, ovary, and fetal kidney. Moderate amounts in ***** liver, kidney, pancreas, spleen, and fetal lung, and small amounts in placenta, ***** lung, prostate, testis, colon, fetal brain and liver.
Certificate of Analysis
A 1:1000 dilution was sufficient for detection of FZD4 in 15 ug of mouse Testes cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Product Notes
Conjugated primary antibodies can be good options or alternatives to secondary antibody's for detecting a target protein. The lab manufactures a conjugated antibody from a primary parent antibody by attaching (conjugating) an available tag such as a fluorophore or protein, allowing detection of the target protein without using a secondary antibody. Conjugated primary antibodies may potentially be used to replace primary/secondary antibody pairs in various applications, such as Enzyme-Linked Immunosorbent Assay (ELISA), Flow Cytometry, Fluorescence-activated cell sorting, F?rster Resonance Energy Transfer (FRET). Immunocytochemistry, Immunofluorescence, Immunohistochemistry, or Western Blot. The suitability of applications for conjugates are user determined and we cannot guarantee that any antibody-conjugate combination has been tested for use in a particular application.
Please note: Conjugate tags may potentially bind in the antibody paratope, which can limit binding of the antibody to the antigen. Binding in the antibody paratope may affect the ability of the antibody to bind to the corresponding antigen (protein target) in various species and applications. There is no way to confirm or guarantee the location of the antibody tag as we cannot control the binding of the conjugate to the antibody. Researchers are responsible for evaluating antibody conjugates for the suitability of their applications.
Preparation and Storage
Store at-20 degree C.
Other Notes
Small volumes of anti-FZD4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-FZD4 antibody
Cell Signaling; Epigenetics and Nuclear Signaling; Transcription; Domain Families; Stem Cells; Signaling Pathways; Wnt/Beta Catenin Pathway; Cancer; Tumor Suppressors
Applications Tested/Suitable for anti-FZD4 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-FZD4 antibody
WB: 1:1000
IHC: 1:50
Immunohistochemistry (IHC) of anti-FZD4 antibody
Immunohistochemistry analysis using Rabbit Anti-FZD4 Polyclonal Antibody. Tissue: Kidney. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-FZD4 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse testis lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-FZD4 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~59.9 kDa.
NCBI/Uniprot data below describe general gene information for FZD4. It may not necessarily be applicable to this product.
NCBI Accession #
NP_036325.2
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NCBI GenBank Nucleotide #
NP_036325.2
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UniProt Primary Accession #
Q9ULV1
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UniProt Related Accession #
Q9ULV1[Other Products]
NCBI Official Full Name
frizzled-4
NCBI Official Synonym Full Names
frizzled class receptor 4
NCBI Official Symbol
FZD4??[Similar Products]
NCBI Official Synonym Symbols
Fz4; EVR1; FEVR; Fz-4; FzE4; GPCR; hFz4; CD344; FZD4S
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NCBI Protein Information
frizzled-4
UniProt Protein Name
Frizzled-4
UniProt Synonym Protein Names
FzE4
UniProt Gene Name
FZD4??[Similar Products]
UniProt Synonym Gene Names
Fz-4; hFz4??[Similar Products]
UniProt Entry Name
FZD4_HUMAN
NCBI Summary for FZD4
This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This protein may play a role as a positive regulator of the Wingless type MMTV integration site signaling pathway. A transcript variant retaining intronic sequence and encoding a shorter isoform has been described, however, its expression is not supported by other experimental evidence. [provided by RefSeq, Jul 2008]
UniProt Comments for FZD4
FZD4: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes. Plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin (NDP). In retina, it can be both activated by Wnt protein-binding, but also by a Wnt-independent signaling via binding of norrin (NDP), promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LEF/TCF-mediated transcriptional programs. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Defects in FZD4 are the cause of vitreoretinopathy exudative type 1 (EVR1); also known as autosomal dominant familial exudative vitreoretinopathy (FEVR) or Criswick- Schepens syndrome. EVR1 is a disorder of the retinal vasculature characterized by an abrupt cessation of growth of peripheral capillaries, leading to an avascular peripheral retina. This may lead to compensatory retinal neovascularization, which is thought to be induced by hypoxia from the initial avascular insult. New vessels are prone to leakage and rupture causing exudates and bleeding, followed by scarring, retinal detachment and blindness. Clinical features can be highly variable, even within the same family. Patients with mild forms of the disease are asymptomatic, and their only disease-related abnormality is an arc of avascular retina in the extreme temporal periphery. Belongs to the G-protein coupled receptor Fz/Smo family.
Protein type: Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral; GPCR, Fz/Smo family
Chromosomal Location of Human Ortholog: 11q14.2
Cellular Component: cell surface; integral to plasma membrane; integral to membrane; plasma membrane; intercellular junction
Molecular Function: G-protein coupled receptor activity; Wnt-protein binding; Wnt receptor activity; protein binding; protein homodimerization activity; protein heterodimerization activity; cytokine binding; ubiquitin protein ligase binding; PDZ domain binding
Biological Process: positive regulation of JNK activity; Wnt receptor signaling pathway; positive regulation of transcription, DNA-dependent; Wnt receptor signaling pathway through beta-catenin; progesterone secretion; locomotion during locomotory behavior; neuron differentiation; G-protein coupled receptor protein signaling pathway; Wnt receptor signaling pathway, calcium modulating pathway; sensory perception of sound; regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of transcription factor activity; vasculogenesis
Disease: Exudative Vitreoretinopathy 1
Research Articles on FZD4
1. these results demonstrate that during the course of evolution, FZD4 has acquired new functions or epistasis via complex patter of gene duplications, sequence divergence and conformational remodeling.
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