KIAA0589; LCCS3; PlP5K-GAMMA; PlP5Kgamma; PlPKlg_v4

For Research Use Only. Not for use in diagnostic procedures.

Sf9 cells

>80%

Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM, DTT, 0.1mM PMSF, and 25% glycerol.

0.1 mg/ml (lot specific)

Ships on dry ice. Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Small volumes of PIP5K1C recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Description: Recombinant full-length human PIP5K1C was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Scientific Background: PIP5K1C or phosphatidylinositol-4-phosphate 5-kinase 1 gamma phosphorylates phosphatidylinositol-4-phosphate to form phosphatidylinositol 4,5-bisphosphate. PIP5K1C is found at synapses and has been found to play roles in endocytosis and cell migration. PIP5K1C directly associates with beta-catenin and increases beta-catenin activity downstream of growth factor stimulation (1). PIP5K1C expression and kinase activity enhance beta-catenin phosphorylation on residues that promote nuclear importation and transcriptional activity. PIP5K1C deficiency impairs neutrophil recruitment as a result of defect in adhesion. PIP5K1C regulated the adhesion through facilitating RhoA GTPase and integrin activation by chemoattractants (2).

Enzymes; Active Lipid Kinases

The specific activity of PIP5K1C was determined to be 6,927 nmol/min/mg as per activity assay protocol.

The purity of PIP5K1C was detrermined to be >70% by densitometry approx. MW 120 kDa.

phosphatidylinositol-4-phosphate 5-kinase type 1 gamma

phosphatidylinositol 4-phosphate 5-kinase type-1 gamma

Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma

KIAA0589; PIP5K1-gamma; PtdIns(4)P-5-kinase 1 gamma; PIP5KIgamma??[Similar Products]

This locus encodes a type I phosphatidylinositol 4-phosphate 5-kinase. The encoded protein catalyzes phosphorylation of phosphatidylinositol 4-phosphate, producing phosphatidylinositol 4,5-bisphosphate. This enzyme is found at synapses and has been found to play roles in endocytosis and cell migration. Mutations at this locus have been associated with lethal congenital contractural syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Sep 2010]

PIP5K1C: a member of the type I phosphatidylinositol-4-phosphate 5-kinase family of enzymes. A similar protein in mice is found in synapses and focal adhesion plaques, and binds the FERM domain of talin through its C-terminus.

Protein type: Carbohydrate Metabolism - inositol phosphate; EC 2.7.1.68; Kinase, lipid; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cytosol; focal adhesion; nucleoplasm; uropod

Molecular Function: 1-phosphatidylinositol-4-phosphate 5-kinase activity; protein binding

Biological Process: actin cytoskeleton organization and biogenesis; cell-cell adhesion; neutrophil chemotaxis; phagocytosis; phosphatidylinositol biosynthetic process; regulation of phosphoinositide 3-kinase cascade; synaptic vesicle endocytosis; synaptic vesicle exocytosis

Disease: Lethal Congenital Contracture Syndrome 3

1. Schramp M. et al: PIPKIgamma regulates beta-catenin transcriptional activity downstream of growth factor receptor signaling. Cancer Res. 2011 Feb 15;71 (4):1282-91. 2. Xu W, Integrin-induced PIP5K1C kinase polarization regulates neutrophil polarization, directionality, and in vivo infiltration. Immunity. 2010 Sep 24;33 (3):340-50.

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