Full Product Name
PTRF antibody
Product Synonym Names
Polyclonal PTRF; Anti-PTRF
Product Gene Name
anti-PTRF antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O54724
Species Reactivity
Mouse, Rat, Dog, Human, Bovine
Form/Format
Lyophilized powder. Add 100 ul of distilled water. Final antibody concentration is 1 mg/ml in PBS buffer.
Concentration
1 mg/ml (lot specific)
Biological Significance
In mouse, pausing of elongation complexes is mediated by the transcription termination factor TTF-I bound to the 'Sal box' terminator downstream of the rDNA transcription unit. PTRF is a Pol I and transcript release factor for dissociation of paused ternary complexes.
Immunogen
PTRF antibody was raised in rabbit using the middle region of PTRF as the immunogen
Preparation and Storage
Store at 4 degree C, following reconstitution, aliquot and store at -20 degree C. Avoid repeated freeze-thaw cycles.
Other Notes
Small volumes of anti-PTRF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PTRF antibody
Rabbit polyclonal PTRF antibody
Product Categories/Family for anti-PTRF antibody
DNA & RNA
Applications Tested/Suitable for anti-PTRF antibody
Western Blot (WB)
Application Notes for anti-PTRF antibody
WB: 0.625 ug/ml
Western Blot (WB) of anti-PTRF antibody
Recommended PTRF Antibody Titration: 0.625ug/ml
Immunohistochemistry (IHC) of anti-PTRF antibody
Paraffin embedded mouse vessel and adipocytes (skeletal muscle) tissue, tested with an antibody Dilution of 5 ug/ml.
Western Blot (WB) of anti-PTRF antibody
Lane 1: 50ug mouse embryonic fibroblast lysate Lane 2: 50ug mouse PTRF-KO embryonic fibroblast lysate Lane 3: 50ug mouse PTRF-KO embryonic fibroblast lysate+FLAG-PTRF protein Primary Antibody Dilution: 1:1000 Secondary Antibody: Donkey anti-Rabbit-HRP Secondary Antibody Dilution: 1:10000
NCBI/Uniprot data below describe general gene information for PTRF. It may not necessarily be applicable to this product.
NCBI Accession #
NP_033012
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NCBI GenBank Nucleotide #
NM_008986.2
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UniProt Primary Accession #
O54724
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UniProt Secondary Accession #
Q2TAW6[Other Products]
UniProt Related Accession #
O54724[Other Products]
Molecular Weight
43,954 Da
NCBI Official Full Name
polymerase I and transcript release factor
NCBI Official Synonym Full Names
polymerase I and transcript release factor
NCBI Official Symbol
Ptrf??[Similar Products]
NCBI Official Synonym Symbols
Cavin; Cav-p60; AW546441; 2310075E07Rik
??[Similar Products]
NCBI Protein Information
polymerase I and transcript release factor
UniProt Protein Name
Polymerase I and transcript release factor
UniProt Synonym Protein Names
Cav-p60; Cavin-1
Protein Family
Polymerase I and transcript release factor
UniProt Gene Name
Ptrf??[Similar Products]
UniProt Entry Name
PTRF_MOUSE
UniProt Comments for PTRF
PTRF: Plays an important role in caveolae formation and organization. Required for the sequestration of mobile caveolin into immobile caveolae. Termination of transcription by RNA polymerase I involves pausing of transcription by TTF1, and the dissociation of the transcription complex, releasing pre-rRNA and RNA polymerase I from the template. PTRF is required for dissociation of the ternary transcription complex. Defects in PTRF are the cause of congenital generalized lipodystrophy type 4 (CGL4). It is a disorder characterized by the association of congenital generalized lipodystrophy with muscular dystrophy and cardiac anomalies. Congenital generalized lipodystrophy is characterized by a near complete absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes. Belongs to the PTRF/SDPR family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription initiation complex
Cellular Component: protein complex; mitochondrion; intracellular membrane-bound organelle; membrane; endoplasmic reticulum; cytoplasm; plasma membrane; caveola; nucleus
Molecular Function: rRNA binding; rRNA primary transcript binding; RNA binding
Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; transcription initiation from RNA polymerase I promoter; termination of RNA polymerase I transcription; transcription termination
Research Articles on PTRF
1. these results suggested that the membrane dynamics in cell migration is affected by caveolae associated PTRF/cavin-1.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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