Product Name
Frizzled-4 (Fz-4), Polyclonal Antibody
Full Product Name
Rabbit Anti-Human Frizzled-4 Polyclonal Antibody
Product Synonym Names
Frizzled-4; hFz4; FzE4; CD antigen CD344
Product Gene Name
anti-Fz-4 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9ULV1
Specificity
The antibody detects endogenous Frizzled-4
Purity/Purification
>=95% by SDS-PAGE
Affinity purified by Protein A.
Form/Format
Lyophilized or Liquid
Concentration
1 mg/mL (lot specific)
Immunogen
Synthetic peptide from human protein at AA range: 11-60
Storage Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Preparation and Storage
Upon delivery aliquot and store at -20 degree C for one year. Avoid repeated freeze/thaw cycles.
The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20 degree C.
When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-8 degree C.
Other Notes
Small volumes of anti-Fz-4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-Fz-4 antibody
Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
Application Notes for anti-Fz-4 antibody
IHC-p: 1:50-200
ELISA: 1:10000-20000
NCBI/Uniprot data below describe general gene information for Fz-4. It may not necessarily be applicable to this product.
NCBI Accession #
NP_036325.2
[Other Products]
NCBI GenBank Nucleotide #
NM_012193.3
[Other Products]
UniProt Primary Accession #
Q9ULV1
[Other Products]
UniProt Secondary Accession #
Q14C97; Q6S9E4; A8K9Q3[Other Products]
UniProt Related Accession #
Q9ULV1[Other Products]
Molecular Weight
59,881 Da
NCBI Official Full Name
frizzled-4
NCBI Official Synonym Full Names
frizzled class receptor 4
NCBI Official Symbol
FZD4??[Similar Products]
NCBI Official Synonym Symbols
Fz4; EVR1; FEVR; Fz-4; FzE4; GPCR; hFz4; CD344; FZD4S
??[Similar Products]
NCBI Protein Information
frizzled-4
UniProt Protein Name
Frizzled-4
UniProt Synonym Protein Names
FzE4; CD_antigen: CD344
UniProt Gene Name
FZD4??[Similar Products]
UniProt Synonym Gene Names
Fz-4; hFz4??[Similar Products]
NCBI Summary for Fz-4
This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This protein may play a role as a positive regulator of the Wingless type MMTV integration site signaling pathway. A transcript variant retaining intronic sequence and encoding a shorter isoform has been described, however, its expression is not supported by other experimental evidence. [provided by RefSeq, Jul 2008]
UniProt Comments for Fz-4
Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes. Plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin (NDP). In retina, it can be both activated by Wnt protein-binding, but also by a Wnt-independent signaling via binding of norrin (NDP), promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LEF/TCF-mediated transcriptional programs. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
Research Articles on Fz-4
1. Seven novel mutations found in this study have broadened the spectrum of mutations in FZD4 known to cause familial exudative vitreoretinopathy (FEVR), providing a deeper understanding of this disease. The results show that mutations in FZD4 are associated with the phenotypes of retinal folds or ectopic macula in FEVR
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
