Product Name
Wilms Tumor Protein (WT1), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Wilms Tumor Protein (WT1)
Product Synonym Names
WAGR; GUD; WIT-2; WT33
Product Gene Name
anti-WT1 antibody
[Similar Products]
Matching Pairs
Unconjugated Antibody: Wilms Tumor Protein (MBS2033865)
Immunogen: Wilms Tumor Protein (MBS2034154)
Matching Pairs
Unconjugated Antibody: Wilms Tumor Protein (MBS2033865)
APC-CY7 Conjugated Antibody: Wilms Tumor Protein (WT1) (MBS2075973)
Matching Pairs
Unconjugated Antibody: Wilms Tumor Protein (MBS2033865)
PE Conjugated Antibody: Wilms Tumor Protein (WT1) (MBS2075974)
Matching Pairs
Unconjugated Antibody: Wilms Tumor Protein (MBS2033865)
APC Conjugated Antibody: Wilms Tumor Protein (WT1) (MBS2075975)
Matching Pairs
Unconjugated Antibody: Wilms Tumor Protein (MBS2033865)
Cy3 Conjugated Antibody: Wilms Tumor Protein (WT1) (MBS2075976)
Matching Pairs
Unconjugated Antibody: Wilms Tumor Protein (MBS2033865)
FITC Conjugated Antibody: Wilms Tumor Protein (WT1) (MBS2075977)
Matching Pairs
Unconjugated Antibody: Wilms Tumor Protein (MBS2033865)
HRP Conjugated Antibody: Wilms Tumor Protein (WT1) (MBS2075978)
Matching Pairs
Unconjugated Antibody: Wilms Tumor Protein (MBS2033865)
APC-CY7 Conjugated Secondary Antibody: Immunoglobulin G (MBS2090675)
Matching Pairs
Unconjugated Antibody: Wilms Tumor Protein (MBS2033865)
Unconjugated Secondary Antibody: Immunoglobulin G (MBS2090678)
Matching Pairs
Unconjugated Antibody: Wilms Tumor Protein (MBS2033865)
Biotin Conjugated Antibody: Wilms Tumor Protein (WT1) (MBS2095900)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P19544
Specificity
The antibody is a rabbit polyclonal antibody raised against WT1. It has been selected for its ability to recognize WT1 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, Containing 0.02% NaN3, 50% glycerol.
Concentration
200ug/ml (lot specific)
Fragment
WT1 (Leu310~Leu449)
Quality Control
Content: The quality control contains recombinant WT1 (Leu310~Leu449) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2075975
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.
Other Notes
Small volumes of anti-WT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-WT1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-WT1 antibody
Western blotting: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Western Blot (WB) of anti-WT1 antibody
Western Blot: Sample: Recombinant protein.
Immunohistochemistry (IHC) of anti-WT1 antibody
DAB staining on fromalin fixed paraffin- embedded kidney tissue)
NCBI/Uniprot data below describe general gene information for WT1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000369.3
[Other Products]
NCBI GenBank Nucleotide #
NM_000378.4
[Other Products]
UniProt Primary Accession #
P19544
[Other Products]
UniProt Secondary Accession #
Q15881; Q16256; Q16575; Q4VXV4; Q4VXV5; Q4VXV6; Q8IYZ5; A8K6S1; B3KSA5[Other Products]
UniProt Related Accession #
P19544[Other Products]
Molecular Weight
33,086 Da
NCBI Official Full Name
Wilms tumor protein isoform A
NCBI Official Synonym Full Names
Wilms tumor 1
NCBI Official Symbol
WT1??[Similar Products]
NCBI Official Synonym Symbols
GUD; AWT1; WAGR; WT33; NPHS4; WIT-2; EWS-WT1
??[Similar Products]
NCBI Protein Information
Wilms tumor protein
UniProt Protein Name
Wilms tumor protein
UniProt Synonym Protein Names
WT33
Protein Family
Wilms tumor protein
UniProt Gene Name
WT1??[Similar Products]
NCBI Summary for WT1
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015]
UniProt Comments for WT1
Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:7862533, PubMed:17716689, PubMed:25258363). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921).
Research Articles on WT1
1. Data reveal that MiR-193a directly targets WT1 and indirectly modulates E-cadherin and that overexpression of WT1 partially prevents miR-193a-induced inhibition of migration and invasion of non-small cell lung cancers, suggesting that WT1 plays an important role in the anti-metastasis by miR-193a.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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