Product Name
Wilms Tumor Protein (WT1), Monoclonal Antibody
Full Product Name
Wilms Tumor Protein
Product Synonym Names
Wilms tumor protein; WT33
Product Gene Name
anti-WT1 antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P19544
Species Reactivity
Human, Mouse
Purity/Purification
ProA affinity purified
Immunogen
Recombinant Protein
Storage Buffer
1*TBS (pH7.4), 1% BSA, 40% Glycerol.
Preservative
0.05% Sodium Azide.
Preparation and Storage
Aliquot and store at-20 degree C. Avoid repeated freeze/thaw cycles.
Other Notes
Small volumes of anti-WT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-WT1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
NCBI/Uniprot data below describe general gene information for WT1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000369.3
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NCBI GenBank Nucleotide #
NM_000378.4
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UniProt Primary Accession #
P19544
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UniProt Secondary Accession #
Q15881; Q16256; Q16575; Q4VXV4; Q4VXV5; Q4VXV6; Q8IYZ5; A8K6S1; B3KSA5[Other Products]
UniProt Related Accession #
P19544[Other Products]
Molecular Weight
33,086 Da
NCBI Official Full Name
Wilms tumor protein isoform A
NCBI Official Synonym Full Names
Wilms tumor 1
NCBI Official Symbol
WT1??[Similar Products]
NCBI Official Synonym Symbols
GUD; AWT1; WAGR; WT33; NPHS4; WIT-2
??[Similar Products]
NCBI Protein Information
Wilms tumor protein
UniProt Protein Name
Wilms tumor protein
UniProt Synonym Protein Names
WT33
Protein Family
Wilms tumor protein
UniProt Gene Name
WT1??[Similar Products]
NCBI Summary for WT1
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015]
UniProt Comments for WT1
Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:7862533, PubMed:17716689, PubMed:25258363). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921).
Research Articles on WT1
1. in vitro evidence to demonstrate that Wilms' tumor 1 (WT1) negatively regulates the expression of the atRA synthetic enzymes, ALDH1A1, ALDH1A2 and ALDH1A3, in the 293 cell line, leading to significant blockage of atRA production.
Precautions
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Disclaimer
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