For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Mouse WT1. No significant cross-reactivity or interference between Mouse WT1 and analogues was observed.

Store at 2-8 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of WT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS280490 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Wilms tumor protein (WT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing WT1. The ELISA analytical biochemical technique of the MBS280490 kit is based on WT1 antibody-WT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate WT1 in samples. An antibody specific for WT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

54,943 Da

Wilms tumor 1 homolog

Wilms tumor protein homolog

Wilms tumor protein homolog

Wt-1??[Similar Products]

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It plays an essential role in the normal development of the urogenital system, and the orthologous human gene is mutated in a small subset of patients with Wilm's tumors. Alternative splicing has been noted for this gene, however, the full-length nature of these variants is not known. The mRNA for this gene has been shown to initiate translation from non-AUG (CUG) and AUG translation start sites, resulting in different isoforms. [provided by RefSeq, Apr 2013]

WT1: a DNA binding protein and apparent transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. Expressed in the kidney and a subset of hematopoietic cells. Defects in WT1 are the cause of Wilms tumor 1 (WT1), an embryonal malignancy of the kidney that affects approximately 1 in 10'000 infants and young children. It occurs both in sporadic and hereditary forms. Four alternatively spliced isoforms have been described.

Protein type: C2H2-type zinc finger protein; DNA-binding; Nucleolus; Transcription factor; Tumor suppressor

Chromosomal Location of Human Ortholog: 2 E2-E3|2 55.06 cM

Cellular Component: cytoplasm; cytosol; nuclear speck; nucleoplasm; nucleus

Molecular Function: DNA binding; DNA binding transcription factor activity; double-stranded DNA binding; double-stranded methylated DNA binding; hemi-methylated DNA-binding; metal ion binding; nucleic acid binding; protein binding; RNA binding; sequence-specific DNA binding; transcription regulatory region DNA binding; zinc ion binding

Biological Process: adrenal gland development; apoptosis; branching involved in ureteric bud morphogenesis; camera-type eye development; cellular response to gonadotropin stimulus; epithelial cell differentiation; germ cell development; glomerular basement membrane development; glomerular visceral epithelial cell development; glomerular visceral epithelial cell differentiation; glomerulus development; gonad development; heart development; kidney development; male genitalia development; male gonad development; mesonephros development; metanephric comma-shaped body morphogenesis; metanephric mesenchyme development; metanephric S-shaped body morphogenesis; metanephros development; negative regulation of apoptosis; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of female gonad development; negative regulation of mesenchymal cell apoptotic process involved in metanephros development; negative regulation of metanephric glomerular mesangial cell proliferation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of translation; positive regulation of apoptosis; positive regulation of male gonad development; positive regulation of metanephric ureteric bud development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; posterior mesonephric tubule development; regulation of animal organ formation; regulation of gene expression; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; RNA splicing; seminiferous tubule development; Sertoli cell development; sex determination; single fertilization; sperm motility; spermatogenesis; thorax and anterior abdomen determination; tissue development; transcription, DNA-dependent; ureteric bud development; vasculogenesis

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