Product Name
Wilms Tumor Protein (WT1), Polyclonal Antibody
Full Product Name
Anti-Wilms Tumor Protein Rabbit pAb
Product Synonym Names
GUD; AWT1; WAGR; WT33; NPHS4; WIT-2; EWS-WT1
Product Gene Name
anti-WT1 antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P19544
Species Reactivity
Human, Mouse
Specificity
Wilms Tumor Protein Antibody detects endogenous levels of total Wilms Tumor Protein
Purity/Purification
Affinity Chromatography
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
A synthesized peptide derived from human Wilms Tumor Protein
Subcellular Location
Nucleus
Preparation and Storage
Store at 4 degree C for short term.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.
Other Notes
Small volumes of anti-WT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-WT1 antibody
Has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm' s tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG.
Applications Tested/Suitable for anti-WT1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes for anti-WT1 antibody
WB: 1:500-1:1000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
FC/FACS: 1:50
Western Blot (WB) of anti-WT1 antibody
Western blot analysis of WT1 expression in K562 cell lysate.
Immunohistochemistry (IHC) of anti-WT1 antibody
Immunohistochemical analysis of paraffin-embedded mouse kidney, using Wilms Tumor Protein Antibody.
NCBI/Uniprot data below describe general gene information for WT1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000369.3
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NCBI GenBank Nucleotide #
NM_000378.4
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UniProt Primary Accession #
P19544
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UniProt Secondary Accession #
Q15881; Q16256; Q16575; Q4VXV4; Q4VXV5; Q4VXV6; Q8IYZ5; A8K6S1; B3KSA5[Other Products]
UniProt Related Accession #
P19544[Other Products]
Molecular Weight
55kDa[Similar Products]
NCBI Official Full Name
Wilms tumor protein isoform A
NCBI Official Synonym Full Names
Wilms tumor 1
NCBI Official Symbol
WT1??[Similar Products]
NCBI Official Synonym Symbols
GUD; AWT1; WAGR; WT33; NPHS4; WIT-2
??[Similar Products]
NCBI Protein Information
Wilms tumor protein
UniProt Protein Name
Wilms tumor protein
UniProt Synonym Protein Names
WT33
Protein Family
Wilms tumor protein
UniProt Gene Name
WT1??[Similar Products]
NCBI Summary for WT1
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015]
UniProt Comments for WT1
Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:7862533, PubMed:17716689, PubMed:25258363). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921).
Research Articles on WT1
1. in vitro evidence to demonstrate that Wilms' tumor 1 (WT1) negatively regulates the expression of the atRA synthetic enzymes, ALDH1A1, ALDH1A2 and ALDH1A3, in the 293 cell line, leading to significant blockage of atRA production.
Precautions
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