Anti -ADRA2A (Alpha-2A Adrenergic Receptor, Alpha-2 Adrenergic Receptor Subtype C10, Alpha-2A Adrenoreceptor, Alpha-2A Adrenoceptor, Alpha-2AAR, ADRA2R, ADRAR)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 10; NC_000010.10 (112836790..112840665). Location: 10q24-q26

Polyclonal

IgG

Rabbit

Recognizes human ADRA2. Species Cross-Reactivity: mouse, rat, rabbit.

Affinity Purified
Purified by immunoaffinity chromatography.

Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with sterile dH2O.

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile dH20. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-ADRA2A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Alpha-2-adrenergic receptor (ADRA2), also known as platelet type adrenoceptor alpha-2A, is a member of G protein-coupled receptor superfamily. This gene, which can identify a DraI RFLP of the ADRAR gene, mapped to 10q23-q25, is found in the distal region of mouse chromosome 19 and abundantly expressed in giant cell. ADRA2 acts a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. It has 3 highly homologous subtypes: ADRA2A; ADRA2B and ADRA2C. Studies in mouse suggested that both the ADRA2A and ADRA2C subtypes are required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons. ADRA2A receptors inhibited transmitter release at high stimulation frequencies, whereas the ADRA2C subtype modulated neurotransmission at lower levels of nerve activity.

Antibodies; Abs to Receptors

Western Blot (WB), Immunohistochemistry (IHC)

Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 1ug/ml
Immunohistochemistry (paraffin): 1-2ug/ml, boiling the sections is required.

48,957 Da[Similar Products]

adrenergic, alpha-2A-, receptor

alpha-2A adrenergic receptor; OTTHUMP00000020485; alpha-2A adrenoceptor; alpha-2AAR subtype C10; alpha-2A adrenoreceptor; alpha-2 adrenergic receptor subtype C10; alpha-2-adrenergic receptor, platelet type

Alpha-2A adrenergic receptor

ADRA2R; ADRAR??[Similar Products]

ADA2A_HUMAN

Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Studies in mouse revealed that both the alpha2A and alpha2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons; the alpha2A subtype inhibited transmitter release at high stimulation frequencies, whereas the alpha2C subtype modulated neurotransmission at lower levels of nerve activity. This gene encodes alpha2A subtype and it contains no introns in either its coding or untranslated sequences. [provided by RefSeq]

ADRA2A: Alpha-2 adrenergic receptors mediate the catecholamine- induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is oxymetazoline > clonidine > epinephrine > norepinephrine > phenylephrine > dopamine > p-synephrine > p-tyramine > serotonin = p-octopamine. For antagonists, the rank order is yohimbine > phentolamine = mianserine > chlorpromazine = spiperone = prazosin > propanolol > alprenolol = pindolol. Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA2A sub-subfamily.

Protein type: GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q25.2

Cellular Component: integral to plasma membrane; basolateral plasma membrane; cytoplasm; plasma membrane; synapse; receptor complex

Molecular Function: protein binding; protein homodimerization activity; alpha-2C adrenergic receptor binding; protein heterodimerization activity; thioesterase binding; alpha2-adrenergic receptor activity; alpha-1B adrenergic receptor binding; norepinephrine binding; epinephrine binding; protein kinase binding; heterotrimeric G-protein binding

Biological Process: fear response; negative regulation of calcium ion-dependent exocytosis; positive regulation of potassium ion transport; female pregnancy; thermoception; positive regulation of vasodilation; signal transduction; glucose homeostasis; positive regulation of membrane protein ectodomain proteolysis; Rho protein signal transduction; intestinal absorption; negative regulation of insulin secretion; positive regulation of MAP kinase activity; negative regulation of cAMP biosynthetic process; positive regulation of epidermal growth factor receptor activity; negative regulation of lipid catabolic process; positive regulation of cell proliferation; DNA replication; positive regulation of cytokine production; platelet activation; negative regulation of calcium ion transport; activation of protein kinase B; negative regulation of adenylate cyclase activity; cellular response to hormone stimulus; G-protein coupled receptor protein signaling pathway; regulation of vasoconstriction; Ras protein signal transduction; activation of protein kinase activity; energy reserve metabolic process; negative regulation of epinephrine secretion; cell motility; actin cytoskeleton organization and biogenesis; blood coagulation; regulation of insulin secretion; acute inflammatory response; positive regulation of cell migration

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