For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Small volumes of anti-ADRA2A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Polyclonal

Western Blot (WB)

Western blot analysis of extracts of various cell lines, using ADRA2A antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

207

adrenoceptor alpha 2A

alpha-2A adrenergic receptor; alpha-2A adrenoceptor; alpha-2AAR subtype C10; alpha-2A adrenoreceptor; adrenergic, alpha-2A-, receptor; alpha-2 adrenergic receptor subtype C10; alpha-2-adrenergic receptor, platelet type

Alpha-2A adrenergic receptor

ADRA2R; ADRAR; Alpha-2A adrenoceptor; Alpha-2AAR??[Similar Products]

ADA2A_HUMAN

Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Studies in mouse revealed that both the alpha2A and alpha2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons; the alpha2A subtype inhibited transmitter release at high stimulation frequencies, whereas the alpha2C subtype modulated neurotransmission at lower levels of nerve activity. This gene encodes alpha2A subtype and it contains no introns in either its coding or untranslated sequences. [provided by RefSeq, Jul 2008]

ADRA2A: Alpha-2 adrenergic receptors mediate the catecholamine- induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is oxymetazoline > clonidine > epinephrine > norepinephrine > phenylephrine > dopamine > p-synephrine > p-tyramine > serotonin = p-octopamine. For antagonists, the rank order is yohimbine > phentolamine = mianserine > chlorpromazine = spiperone = prazosin > propanolol > alprenolol = pindolol. Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA2A sub-subfamily.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR

Chromosomal Location of Human Ortholog: 10q25.2

Cellular Component: basolateral plasma membrane; integral to plasma membrane; cytoplasm; plasma membrane; synapse; receptor complex

Molecular Function: protein binding; protein homodimerization activity; alpha-2C adrenergic receptor binding; protein heterodimerization activity; alpha2-adrenergic receptor activity; thioesterase binding; alpha-1B adrenergic receptor binding; norepinephrine binding; epinephrine binding; protein kinase binding; heterotrimeric G-protein binding

Biological Process: fear response; negative regulation of calcium ion-dependent exocytosis; positive regulation of potassium ion transport; thermoception; female pregnancy; glucose homeostasis; positive regulation of vasodilation; signal transduction; positive regulation of membrane protein ectodomain proteolysis; Rho protein signal transduction; negative regulation of insulin secretion; intestinal absorption; positive regulation of MAP kinase activity; negative regulation of cAMP biosynthetic process; negative regulation of lipid catabolic process; positive regulation of epidermal growth factor receptor activity; positive regulation of cell proliferation; DNA replication; positive regulation of cytokine production; platelet activation; negative regulation of calcium ion transport; activation of protein kinase B; negative regulation of adenylate cyclase activity; cellular response to hormone stimulus; G-protein coupled receptor protein signaling pathway; regulation of vasoconstriction; Ras protein signal transduction; activation of protein kinase activity; negative regulation of epinephrine secretion; energy reserve metabolic process; actin cytoskeleton organization and biogenesis; blood coagulation; cell motility; regulation of insulin secretion; acute inflammatory response; positive regulation of cell migration

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