Product Name
ADRA2A, siRNA
Full Product Name
ADRA2A siRNA (Rat)
Product Synonym Names
Alpha-2A adrenergic receptor; Alpha-2A adrenoreceptor; Alpha-2A adrenoceptor; Alpha-2AAR; Alpha-2D adrenergic receptor; CA2-47
Product Gene Name
ADRA2A sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P22909
Specificity
ADRA2A siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ADRA2A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ADRA2A sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ADRA2A sirna
siRNA to inhibit ADRA2A expression using RNA interference
Applications Tested/Suitable for ADRA2A sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ADRA2A. It may not necessarily be applicable to this product.
NCBI Accession #
NP_036871.3
[Other Products]
NCBI GenBank Nucleotide #
NM_012739.3
[Other Products]
UniProt Primary Accession #
P22909
[Other Products]
UniProt Related Accession #
P22909[Other Products]
Molecular Weight
48,940 Da
NCBI Official Full Name
alpha-2A adrenergic receptor
NCBI Official Synonym Full Names
adrenoceptor alpha 2A
NCBI Official Symbol
Adra2a??[Similar Products]
NCBI Official Synonym Symbols
RG20; CA2-47; RATRG20
??[Similar Products]
NCBI Protein Information
alpha-2A adrenergic receptor
UniProt Protein Name
Alpha-2A adrenergic receptor
UniProt Synonym Protein Names
Alpha-2A adrenoreceptor; Alpha-2A adrenoceptor; Alpha-2AAR; Alpha-2D adrenergic receptor; CA2-47
Protein Family
Alpha-2A adrenergic receptor
UniProt Gene Name
Adra2a??[Similar Products]
UniProt Synonym Gene Names
Alpha-2A adrenoceptor; Alpha-2AAR??[Similar Products]
UniProt Entry Name
ADA2A_RAT
UniProt Comments for ADRA2A
ADRA2A: Alpha-2 adrenergic receptors mediate the catecholamine- induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is oxymetazoline > clonidine > epinephrine > norepinephrine > phenylephrine > dopamine > p-synephrine > p-tyramine > serotonin = p-octopamine. For antagonists, the rank order is yohimbine > phentolamine = mianserine > chlorpromazine = spiperone = prazosin > propanolol > alprenolol = pindolol. Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA2A sub-subfamily.
Protein type: GPCR, family 1; Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass
Cellular Component: integral to plasma membrane; cytoplasm; plasma membrane; synapse; receptor complex
Molecular Function: protein homodimerization activity; alpha-2C adrenergic receptor binding; adrenoceptor activity; protein heterodimerization activity; thioesterase binding; alpha2-adrenergic receptor activity; norepinephrine binding; epinephrine binding; protein kinase binding; heterotrimeric G-protein binding
Biological Process: regulation of smooth muscle contraction; positive regulation of cytokine production; fear response; platelet activation; negative regulation of calcium ion-dependent exocytosis; activation of protein kinase B; female pregnancy; thermoception; positive regulation of vasodilation; signal transduction; glucose homeostasis; positive regulation of membrane protein ectodomain proteolysis; negative regulation of insulin secretion; cellular response to hormone stimulus; G-protein coupled receptor protein signaling pathway; positive regulation of MAP kinase activity; negative regulation of cAMP biosynthetic process; negative regulation of lipid catabolic process; positive regulation of epidermal growth factor receptor activity; regulation of vasoconstriction; activation of protein kinase activity; DNA replication; acute inflammatory response; positive regulation of cell migration
Research Articles on ADRA2A
1. Dopamine-elicited outward current in subcoeruleus nucleus neurons are mediated by adrenergic alpha2 receptors.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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