Anti -BAF57 (SWI/SNF-related Matrix-associated Actin-dependent Regulator of Chromatin Subfamily E Member 1, BRG1-associated Factor 57, BAF57 SMARCE1)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 17; NC_000017.10 (38781214..38805658, complement). Location: 17q21.2

Polyclonal

Rabbit

Recognizes human BF57. Species Crossreactivity

Affinity Purified
Purified by immunoaffinity chromatography.

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Small volumes of anti-SMARCE1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

BAF57, also known as SMARCE1, is a core subunit of the SWI/SNF complex, which is required for transcriptional activation of genes normally repressed by chromatin. The SWI/SNF chromatin remodeling complex is critical for androgen receptor transcriptional and the BAF57 subunit facilitates direct interaction with the receptor. Studies suggest a blockade of the androgen receptor-BAF57 interaction may target agonisty-induced androgen receptor function. Data also suggest that the SWI/SNF complex is required for transcriptional activation by the estrogen receptor. ChIP assays demonstrated BAF57 is recruited to the estrogen-response promoter, pS2.

Antibodies; Abs to Chromatin Proteins

Western Blot (WB), Immunohistochemistry (IHC)

Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 0.5ug/ml
Immunohistochemistry: 1:300

46,649 Da[Similar Products]

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1; BRG1-associated factor 57; chromatin remodeling complex BRG1-associated factor 57; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1

BAF57; BAF57??[Similar Products]

SMCE1_HUMAN

The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart. [provided by RefSeq, Jul 2008]

SMARCE1: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their ***** state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Required for the coactivation of estrogen responsive promoters by Swi/Snf complexes and the SRC/p160 family of histone acetyltransferases (HATs). Also specifically interacts with the CoREST corepressor resulting in repression of neuronal specific gene promoters in non-neuronal cells. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Defects in SMARCE1 may be a cause of Coffin-Siris syndrome, a highly variable disease characterized by mental retardation associated with a broad spectrum of different clinical features. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: nucleoplasm; nuclear chromosome; SWI/SNF complex; protein complex; transcriptional repressor complex; nuclear chromatin; nucleus

Molecular Function: ligand-dependent nuclear receptor binding; N-acetyltransferase activity; protein binding; nucleosomal DNA binding; RNA binding; transcription coactivator activity; protein N-terminus binding; chromatin binding

Biological Process: regulation of transcription from RNA polymerase II promoter; chromatin remodeling; nervous system development; nucleosome disassembly; metabolic process; ATP-dependent chromatin remodeling; negative regulation of transcription, DNA-dependent

Disease: Meningioma, Familial, Susceptibility To

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