Acyl-CoA-binding domain-containing protein 5; acyl-Coenzyme A binding domain containing 5; endozepine-related protein; membrane-associated diazepam binding inhibitor; ACBD5; KIAA1996

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG

Mouse

Store at -20 degree C. Stable for one year from the date of shipment.

Small volumes of anti-ACBD5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This gene encodes a member of the acyl-Coenzyme A binding protein family, known to function in the transport and distribution of long chain acyl-Coenzyme A in cells. This gene may play a role in the differentiation of megakaryocytes and formation of platelets. A related protein in yeast is involved in autophagy of peroxisomes. A mutation in this gene has been associated with autosomal dominant thrombocytopenia. Alternative splicing results in multiple transcript variants.

Cell Marker; Subcellular Marker; Membrane

Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)

WB: 1:1000-1:5000
IP: 10 tests
FC/FACS: 1:100-1:200

All lanes : ACBD5 Mouse mAb at 1/2000 dilution Lane 1 : Jurkat cells membrane fraction Lane 2 : THP1 cells membrane fraction as negative control Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 60 KDa Observed band size : 60 KDa Blocking/Dilution buffer : 1x TBST.

ACBD5 was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25019 of 10 ug. Western blot was performed from the immunopre-cipitate using M25019 at 1/2000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.

Blocking and dilution buffer and concentration:5% milk/TBST.

Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling ACBD5 with M25019 at 1/200 dilution (blue) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, red). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.

Predicted MW: 60kDa

acyl-CoA binding domain containing 5

acyl-CoA-binding domain-containing protein 5

Acyl-CoA-binding domain-containing protein 5

KIAA1996??[Similar Products]

This gene encodes a member of the acyl-Coenzyme A binding protein family, known to function in the transport and distribution of long chain acyl-Coenzyme A in cells. This gene may play a role in the differentiation of megakaryocytes and formation of platelets. A related protein in yeast is involved in autophagy of peroxisomes. A mutation in this gene has been associated with autosomal dominant thrombocytopenia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Acyl-CoA binding protein which acts as the peroxisome receptor for pexophagy but is dispensable for aggrephagy and nonselective autophagy. Binds medium- and long-chain acyl-CoA esters.

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