Full Product Name
CTRC siRNA (Human)
Product Synonym Names
CLCR; Chymotrypsin-C; Caldecrin
Product Gene Name
CTRC sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q99895
Specificity
CTRC siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CTRC gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CTRC sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CTRC sirna
siRNA to inhibit CTRC expression using RNA interference
Applications Tested/Suitable for CTRC sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CTRC. It may not necessarily be applicable to this product.
NCBI Accession #
NP_009203.2
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NCBI GenBank Nucleotide #
NM_007272.2
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UniProt Primary Accession #
Q99895
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UniProt Secondary Accession #
O00765; Q9NUH5; A8K082[Other Products]
UniProt Related Accession #
Q99895[Other Products]
Molecular Weight
29,484 Da
NCBI Official Full Name
chymotrypsin-C preproprotein
NCBI Official Synonym Full Names
chymotrypsin C (caldecrin)
NCBI Official Symbol
CTRC??[Similar Products]
NCBI Official Synonym Symbols
CLCR; ELA4
??[Similar Products]
NCBI Protein Information
chymotrypsin-C
UniProt Protein Name
Chymotrypsin-C
UniProt Synonym Protein Names
Caldecrin
Protein Family
Chymotrypsin
UniProt Gene Name
CTRC??[Similar Products]
UniProt Synonym Gene Names
CLCR??[Similar Products]
UniProt Entry Name
CTRC_HUMAN
NCBI Summary for CTRC
This gene encodes a member of the peptidase S1 family. The encoded protein is a serum calcium-decreasing factor that has chymotrypsin-like protease activity. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]
UniProt Comments for CTRC
CTRC: Has chymotrypsin-type protease activity and hypocalcemic activity. Genetic variations in CTRC can predispose to chronic pancreatitis by diminishing its protective trypsin-degrading activity. Belongs to the peptidase S1 family. Elastase subfamily.
Protein type: EC 3.4.21.2; Protease
Chromosomal Location of Human Ortholog: 1p36.21
Molecular Function: peptidase activity; serine-type endopeptidase activity
Biological Process: proteolysis
Disease: Pancreatitis, Hereditary
Research Articles on CTRC
1. None of the selected 121 pancreatic cancer samples showed a pancreatitis-predisposing mutation in the CTRC gene.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
