C11; CDR; DD4; CHDR; DD-4; HAKRA; 3-alpha-HSD

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

The antibody detects endogenous levels of total AKR1C4 protein.

Antigen affinity purification.

Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.

1.5 mg/ml (lot specific)

Store at -20 degree C

Small volumes of anti-AKR1C4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver.

Total protein Ab

Western Blot (WB), Immunohistochemistry (IHC)

Western blotting: 1:200-1:1000
Immunohistochemistry: 1:25-1:100

Gel: 8%SDS-PAGE Lysates (from left to right): Mouse liver tissue Amount of lysate: 40ug per lane Primary antibody: 1/400 dilution Secondary antibody dilution: 1/8000 Exposure time: 5 seconds

Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using at dilution 1/30.

37,067 Da

aldo-keto reductase family 1, member C4

aldo-keto reductase family 1 member C4

Aldo-keto reductase family 1 member C4

CHDR; CDR; DD-4; DD4??[Similar Products]

AK1C4_HUMAN

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq, Jul 2008]

AKR1C4: Catalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-alpha- androstan-3-alpha,17-beta-diol (3-alpha-diol). Also has some 20- alpha-hydroxysteroid dehydrogenase activity. The biotransformation of the pesticide chlordecone (kepone) to its corresponding alcohol leads to increased biliary excretion of the pesticide and concomitant reduction of its neurotoxicity since bile is the major excretory route. Defects in AKR1C4 are a cause of 46,XY sex reversal type 8 (SRXY8). A disorder of sex development. Affected individuals have a 46,XY karyotype but present as phenotypically normal females. AKR1C4 mutations may act as modifier of disease severity in SRXY8 patients. A splicing mutation resulting in loss of exon 2 has been found in affected individuals also carrying mutation Val-79 in AKR1C2 (PubMed:21802064). Belongs to the aldo/keto reductase family.

Protein type: Xenobiotic Metabolism - metabolism by cytochrome P450; Oxidoreductase; Lipid Metabolism - primary bile acid biosynthesis; Lipid Metabolism - C21-steroid hormone; EC 1.1.1.225; Lipid Metabolism - androgen and estrogen; EC 1.1.1.357

Chromosomal Location of Human Ortholog: 10p15.1

Cellular Component: cytoplasm; cytosol

Molecular Function: 3(or 17)-alpha-hydroxysteroid dehydrogenase activity; aldo-keto reductase activity; bile acid transmembrane transporter activity; chlordecone reductase activity; electron carrier activity; oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; retinal dehydrogenase activity

Biological Process: androgen metabolic process; bile acid and bile salt transport; bile acid biosynthetic process; bile acid metabolic process; fat-soluble vitamin metabolic process; phototransduction, visible light; retinoid metabolic process; steroid metabolic process; vitamin metabolic process

Disease: 46,xy Sex Reversal 8

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