Aldo-keto reductase family 1 member C4; 111-; 3-alpha-HSD1; 3-alpha-hydroxysteroid dehydrogenase type I; Chlordecone reductase; CDR; Dihydrodiol dehydrogenase 4; DD-4; DD4; HAKRA; AKR1C4; CHDR

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This AKR1C4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11-39 amino acids from the N-terminal region of human AKR1C4.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.5 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-AKR1C4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

AKR1C4 is a member of the aldo/keto reductase
superfamily, which consists of more than 40 known enzymes and
proteins. These enzymes catalyze the conversion of aldehydes and
ketones to their corresponding alcohols by utilizing NADH and/or
NADPH as cofactors. The enzymes display overlapping but distinct
substrate specificity. This enzyme catalyzes the bioreduction of
chlordecone, a toxic organochlorine pesticide, to chlordecone
alcohol in liver. This gene shares high sequence identity with
three other gene members and is clustered with those three genes at
chromosome 10p15-p14.

Cancer; Metabolism; Signal Transduction

Western Blot (WB), ELISA (EIA)

WB~~1:1000

AKR1C4 Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the AKR1C4 antibody detected the AKR1C4 protein (arrow).

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aldo-keto reductase family 1, member C4

aldo-keto reductase family 1 member C4

Aldo-keto reductase family 1 member C4

CHDR; CDR; DD-4; DD4??[Similar Products]

AK1C4_HUMAN

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq, Jul 2008]

AKR1C4: Catalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-alpha- androstan-3-alpha,17-beta-diol (3-alpha-diol). Also has some 20- alpha-hydroxysteroid dehydrogenase activity. The biotransformation of the pesticide chlordecone (kepone) to its corresponding alcohol leads to increased biliary excretion of the pesticide and concomitant reduction of its neurotoxicity since bile is the major excretory route. Defects in AKR1C4 are a cause of 46,XY sex reversal type 8 (SRXY8). A disorder of sex development. Affected individuals have a 46,XY karyotype but present as phenotypically normal females. AKR1C4 mutations may act as modifier of disease severity in SRXY8 patients. A splicing mutation resulting in loss of exon 2 has been found in affected individuals also carrying mutation Val-79 in AKR1C2 (PubMed:21802064). Belongs to the aldo/keto reductase family.

Protein type: Lipid Metabolism - primary bile acid biosynthesis; EC 1.1.1.357; Oxidoreductase; EC 1.1.1.225; Lipid Metabolism - C21-steroid hormone; Xenobiotic Metabolism - metabolism by cytochrome P450; Lipid Metabolism - androgen and estrogen

Chromosomal Location of Human Ortholog: 10p15.1

Cellular Component: cytoplasm; cytosol

Molecular Function: oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; electron carrier activity; aldo-keto reductase activity; chlordecone reductase activity; 3(or 17)-alpha-hydroxysteroid dehydrogenase activity; bile acid transmembrane transporter activity; retinal dehydrogenase activity

Biological Process: bile acid and bile salt transport; steroid metabolic process; bile acid biosynthetic process; phototransduction, visible light; bile acid metabolic process; androgen metabolic process; retinoid metabolic process

Disease: 46,xy Sex Reversal 8

Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812(2010)
Guey, L.T., et al. Eur. Urol. 57(2):283-292(2010)
Li, J., et al. Breast Cancer Res. 12 (2), R19 (2010) :
Hosgood, H.D. III, et al. Respir Med 103(12):1866-1870(2009)
Shen, M., et al. Environ. Mol. Mutagen. 50(4):285-290(2009)

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