Tyrosine-protein kinase SYK; Spleen tyrosine kinase; p72-Syk; SYK

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This Syk Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y525/526 of human Syk.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-SYK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Syk is a positive effector of BCR-stimulated responses. This protein couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-342 and Tyr-346. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion.

Cardiovascular; Immunology; Signal Transduction; Phospho Antibodies

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)

WB~~1:1000

Western blot analysis of anti-Phospho-Syk Pab in mouse liver tissue lysate (35ug/lane). Phospho-Syk(arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

72066

spleen tyrosine kinase

tyrosine-protein kinase SYK

Tyrosine-protein kinase SYK

KSYK_HUMAN

This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Syk: a cytoplasmic tyrosine kinase of the SYK family containing two SH2 domains. Plays a central role in the B cell receptor (BCR) response. An upstream activator of the PI3K, PLCgamma2, and Rac/cdc42 pathways in the BCR response. Required for the sequential events of Fc gamma IIa receptor-mediated phagocytosis. Expression highest in murine spleen, heart, mammary gland and thymus. Two splice variant isoforms have been described.

Protein type: Protein kinase, tyrosine (non-receptor); Protein kinase, TK; Kinase, protein; EC 2.7.10.2; TK group; Syk family

Chromosomal Location of Human Ortholog: 9q22

Cellular Component: T cell receptor complex; extrinsic to internal side of plasma membrane; protein complex; cytoplasm; plasma membrane; cytosol; nucleus; B cell receptor complex

Molecular Function: integrin binding; protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: viral reproduction; transcription factor import into nucleus; positive regulation of interleukin-3 biosynthetic process; positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process; positive regulation of calcium-mediated signaling; regulation of phagocytosis; protein amino acid phosphorylation; activation of JNK activity; B cell receptor signaling pathway; leukocyte adhesion; regulation of transcription factor activity; positive regulation of bone resorption; beta selection; positive regulation of gamma-delta T cell differentiation; angiogenesis; inflammatory response; integrin-mediated signaling pathway; platelet activation; neutrophil chemotaxis; adaptive immune response; regulation of superoxide release; positive regulation of cell adhesion mediated by integrin; positive regulation of mast cell degranulation; regulation of neutrophil degranulation; leukotriene biosynthetic process; cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; organ morphogenesis; neutrophil activation during immune response; serotonin secretion by platelet; leukocyte activation during immune response; macrophage activation during immune response; defense response to bacterium; blood vessel morphogenesis; positive regulation of B cell differentiation; innate immune response; positive regulation of alpha-beta T cell proliferation; lymph vessel development; blood coagulation; positive regulation of alpha-beta T cell differentiation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cytokine secretion

Steinberg, M., et al., Blood 104(3):760-767 (2004). Wang, S., et al., World J. Gastroenterol. 10(12):1815-1818 (2004). Hayes, M.J., et al., Mol. Immunol. 41(4):457-468 (2004). Arndt, P.G., et al., J. Biol. Chem. 279(12):10883-10891 (2004). Ding, Y.B., et al., Zhonghua Yi Xue Za Zhi 84(4):290-293 (2004).

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