Product Name
ADAM22, siRNA
Full Product Name
ADAM22 siRNA (Human)
Product Synonym Names
MDC2; Disintegrin and metalloproteinase domain-containing protein 22; ADAM 22; Metalloproteinase-disintegrin ADAM22-3; Metalloproteinase-like. disintegrin-like. and cysteine-rich protein 2
Product Gene Name
ADAM22 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9P0K1
Specificity
ADAM22 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ADAM22 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ADAM22 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ADAM22 sirna
siRNA to inhibit ADAM22 expression using RNA interference
Applications Tested/Suitable for ADAM22 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ADAM22. It may not necessarily be applicable to this product.
NCBI Accession #
NP_004185.1
[Other Products]
NCBI GenBank Nucleotide #
NM_004194.3
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UniProt Primary Accession #
Q9P0K1
[Other Products]
UniProt Secondary Accession #
O75075; O75076; Q9P0K2; Q9UIA1; Q9UKK2[Other Products]
UniProt Related Accession #
Q9P0K1[Other Products]
Molecular Weight
96,637 Da
NCBI Official Full Name
disintegrin and metalloproteinase domain-containing protein 22 isoform 4 preproprotein
NCBI Official Synonym Full Names
ADAM metallopeptidase domain 22
NCBI Official Symbol
ADAM22??[Similar Products]
NCBI Official Synonym Symbols
MDC2; ADAM 22
??[Similar Products]
NCBI Protein Information
disintegrin and metalloproteinase domain-containing protein 22
UniProt Protein Name
Disintegrin and metalloproteinase domain-containing protein 22
UniProt Synonym Protein Names
Metalloproteinase-disintegrin ADAM22-3; Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 2
Protein Family
Disintegrin and metalloproteinase domain-containing protein
UniProt Gene Name
ADAM22??[Similar Products]
UniProt Synonym Gene Names
MDC2; ADAM 22??[Similar Products]
UniProt Entry Name
ADA22_HUMAN
NCBI Summary for ADAM22
This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of
biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Unlike other members of the ADAM protein family, the protein encoded by this gene lacks metalloprotease activity since it has no zinc-binding motif. This gene is highly expressed in the brain and may function as an integrin ligand in the brain. In mice, it has been shown to be essential for correct myelination in the peripheral nervous system. Alternative splicing results in several transcript variants.[provided by RefSeq, Dec 2010]
UniProt Comments for ADAM22
ADAM22: Probable ligand for integrin in the brain. This is a non catalytic metalloprotease-like protein. Involved in regulation of cell adhesion and spreading and in inhibition of cell proliferation. Neuronal receptor for LGI1. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion
Chromosomal Location of Human Ortholog: 7q21
Cellular Component: axon; integral to membrane
Molecular Function: integrin binding; protein binding; zinc ion binding; metalloendopeptidase activity
Biological Process: central nervous system development; ***** locomotory behavior; negative regulation of cell adhesion; cell adhesion; proteolysis; myelination in the peripheral nervous system
Research Articles on ADAM22
1. Disruption of LGI1-ADAM22 interaction reduces synaptic AMPA receptors in hippocampal neurons.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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