Product Name
DNA excision repair protein ERCC-8 (Ercc8), Recombinant Protein
Full Product Name
Recombinant Mouse DNA excision repair protein ERCC-8 (Ercc8)
Product Gene Name
Ercc8 recombinant protein
[Similar Products]
Product Synonym Gene Name
Ercc8[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
1-397aa, full length protein
Sequence
MLSFLSARQS GLEDPLRLRR AQSTRRVLGL ELNKDRDVER IHGSGVNTLD IEPVEGRYML SGGSDGVVVL YDLENASRQP HYTCKAVCSV GRSHPDVHKY SVETVQWYPH DTGMFTSSSF DKTLKVWDTN TLQAADVFNF EETVYSHHMS PAATKHCLVA VGTRGPKVQL CDLKSGSCSH ILQGHRQEIL AVSWSPRHDY ILATASADSR VKLWDVRRAS GCLLTLDQHN GKKSQAAESA NTAHNGKVNG LCFTSDGLHL LTIGTDNRMR LWNSSSGDNT LVNYGKVCND SRKGLQFAVS CGCSSEFVFV PHGSTIAVYA VHSGERLAML KGHYKSVDCC VFQPNFQELY SGSRDCNILA WVPPSYEPVP DDDDEAPAKS QLNPAFADAW SSSDEDG
3D Structure
ModBase 3D Structure for Q8CFD5
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
>85% (SDS-PAGE) (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Storage Buffer
Tris-based buffer, 50% glycerol
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of Ercc8 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
Ercc8 recombinant protein
This gene encodes a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). CS cells are abnormally sensitive to ultraviolet radiation and are defective in the repair of transcriptionally active genes.
NCBI/Uniprot data below describe general gene information for Ercc8. It may not necessarily be applicable to this product.
NCBI Accession #
NP_082318.2
[Other Products]
NCBI GenBank Nucleotide #
NM_028042.3
[Other Products]
UniProt Primary Accession #
Q8CFD5
[Other Products]
UniProt Secondary Accession #
Q3U066; Q8VDC4; Q9CWK7[Other Products]
UniProt Related Accession #
Q8CFD5[Other Products]
Molecular Weight
43,689 Da
NCBI Official Full Name
DNA excision repair protein ERCC-8
NCBI Official Synonym Full Names
excision repaiross-complementing rodent repair deficiency, complementation group 8
NCBI Official Symbol
Ercc8??[Similar Products]
NCBI Official Synonym Symbols
Csa; Ckn1; 2410022P04Rik; 2810431L23Rik; 4631412O06Rik; B130065P18Rik
??[Similar Products]
NCBI Protein Information
DNA excision repair protein ERCC-8
UniProt Protein Name
DNA excision repair protein ERCC-8
UniProt Synonym Protein Names
Cockayne syndrome WD repeat protein CSA homolog
Protein Family
DNA excision repair protein
UniProt Gene Name
Ercc8??[Similar Products]
UniProt Synonym Gene Names
Ckn1; Csa??[Similar Products]
UniProt Comments for Ercc8
Involved in transcription-coupled nucleotide excision repair. It is required for the recruitment of XAB2, HMGN1 and TCEA1/TFIIS to a transcription-coupled repair complex which removes RNA polymerase II-blocking lesions from the transcribed strand of active genes. It is the substrate-recognition component of the CSA complex (DCX(ERCC8) complex) which promotes the ubiquitination and subsequent proteasomal degradation of ERCC6 in a UV-dependent manner; ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair.
Research Articles on Ercc8
1. This study demonistrated that Cockayne syndrome group A deficiencies predispose to hearing loss and cochlear hair cell degeneration in mice.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
