Anti -MASP1, ID (MASP1, CRARF, CRARF1, PRSS5, Mannan-binding lectin serine protease 1, Complement factor MASP-3, Complement-activating component of Ra-reactive factor, Mannose-binding lectin-associated serine protease 1, Mannose-binding protein-associated serin

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 3; NC_000003.11 (186933873..187009810, complement). Location: 3q27-q28

Polyclonal

IgG

Rabbit

Human

Affinity Purified
Purified by Protein A affinity chromatography.

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Small volumes of anti-MASP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This gene encodes a serine protease that functions as a component of the lectin pathway of complement activation. The complement pathway plays an essential role in the innate and adaptive immune response. The encoded protein is synthesized as a zymogen and is activated when it complexes with the pathogen recognition molecules of lectin pathway, the mannose-binding lectin and the ficolins. This protein is not directly involved in complement activation but may play a role as an amplifier of complement activation by cleaving complement C2 or by activating another complement serine protease, MASP-2. The encoded protein is also able to cleave fibrinogen and factor XIII and may may be involved in coagulation. A splice variant of this gene which lacks the serine protease domain functions as an inhibitor of the complement pathway. Alternate splicing results in multiple transcript variants.

Antibodies; Abs to Complement

ELISA (EL/EIA), Western Blot (WB)

Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500

79,247 Da[Similar Products]

mannan-binding lectin serine peptidase 1 (C4/C2 activating component of Ra-reactive factor)

mannan-binding lectin serine protease 1; serine protease 5; complement factor MASP-3; Ra-reactive factor serine protease p100; mannose-binding protein-associated serine protease; mannose-binding lectin-associated serine protease 1; complement-activating component of Ra-reactive factor

Mannan-binding lectin serine protease 1

CRARF; CRARF1; PRSS5; MASP-1; RaRF??[Similar Products]

MASP1_HUMAN

This gene encodes a serine protease that functions as a component of the lectin pathway of complement activation. The complement pathway plays an essential role in the innate and adaptive immune response. The encoded protein is synthesized as a zymogen and is activated when it complexes with the pathogen recognition molecules of lectin pathway, the mannose-binding lectin and the ficolins. This protein is not directly involved in complement activation but may play a role as an amplifier of complement activation by cleaving complement C2 or by activating another complement serine protease, MASP-2. The encoded protein is also able to cleave fibrinogen and factor XIII and may may be involved in coagulation. A splice variant of this gene which lacks the serine protease domain functions as an inhibitor of the complement pathway. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]

Function: Functions in the lectin pathway of complement, which performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them. The lectin pathway is triggered upon binding of mannan-binding lectin (MBL) and ficolins to sugar moieties which leads to activation of the associated proteases MASP1 and MASP2. Functions as an endopeptidase and may activate MASP2 or C2 or directly activate C3 the key component of complement reaction. Isoform 2 may have an inhibitory effect on the activation of the lectin pathway of complement or may cleave IGFBP5. Ref.5 Ref.24

Enzyme regulation: Inhibited by SERPING1 and A2M. Ref.17 Ref.20

Subunit structure: Homodimer. Interacts with the oligomeric lectins MBL2, FCN2 and FCN3; triggers the lectin pathway of complement through activation of C3. Interacts with SERPING1. Ref.11 Ref.13 Ref.14 Ref.15 Ref.16 Ref.18 Ref.19 Ref.25

Subcellular location: Secreted Ref.5.

Tissue specificity: Protein of the plasma which is primarily expressed by liver. Ref.1 Ref.2 Ref.5 Ref.12

Post-translational modification: The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains

By similarity.N-glycosylated. Some N-linked glycan are of the complex-type

By similarity. Ref.11 Ref.25Autoproteolytic processing of the proenzyme produces the active enzyme composed on the heavy and the light chain held together by a disulfide bond. Isoform 1 but not isoform 2 is activated through autoproteolytic processing.

Involvement in disease: 3MC syndrome 1 (3MC1) [MIM:257920]: A disorder characterized by facial dysmorphism that includes hypertelorism, blepharophimosis, blepharoptosis and highly arched eyebrows, cleft lip and/or palate, craniosynostosis, learning disability and genital, limb and vesicorenal anomalies. The term 3MC syndrome includes Carnevale, Mingarelli, Malpuech, and Michels syndromes.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.26

Sequence similarities: Belongs to the peptidase S1 family.Contains 2 CUB domains.Contains 1 EGF-like domain.Contains 1 peptidase S1 domain.Contains 2 Sushi (CCP/SCR) domains.

Biophysicochemical propertiesKinetic parameters:KM=0.10 mM for Ac-Gly-Lys-OMe (at 30 degrees Celsius) Ref.17 Ref.20KM=310 μM for Bz-Arg-OEt (at 30 degrees Celsius)KM=4.8 μM for C2 (at 37 degrees Celsius)

Sequence caution: The sequence AAH39724.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

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