Product Name
Antithrombin (AT), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Antithrombin (AT)
Product Gene Name
anti-AT antibody
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Matching Pairs
Unconjugated Antibody: Antithrombin (MBS2028437)
APC-CY7 Conjugated Antibody: Antithrombin (AT) (MBS2064841)
Matching Pairs
Unconjugated Antibody: Antithrombin (MBS2028437)
PE Conjugated Antibody: Antithrombin (AT) (MBS2064843)
Matching Pairs
Unconjugated Antibody: Antithrombin (MBS2028437)
APC Conjugated Antibody: Antithrombin (AT) (MBS2064845)
Matching Pairs
Unconjugated Antibody: Antithrombin (MBS2028437)
Cy3 Conjugated Antibody: Antithrombin (AT) (MBS2064847)
Matching Pairs
Unconjugated Antibody: Antithrombin (MBS2028437)
FITC Conjugated Antibody: Antithrombin (AT) (MBS2064849)
Matching Pairs
Unconjugated Antibody: Antithrombin (MBS2028437)
HRP Conjugated Antibody: Antithrombin (AT) (MBS2064851)
Matching Pairs
Unconjugated Antibody: Antithrombin (MBS2028437)
APC-CY7 Conjugated Secondary Antibody: Immunoglobulin G (MBS2090675)
Matching Pairs
Unconjugated Antibody: Antithrombin (MBS2028437)
Unconjugated Secondary Antibody: Immunoglobulin G (MBS2090678)
Matching Pairs
Unconjugated Antibody: Antithrombin (MBS2028437)
Biotin Conjugated Antibody: Antithrombin (AT) (MBS2094396)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- D QIHFFFAKLN CRLYRKANKS SNLVSANRLF GDKSLTFNES YQDVSEIVYG AKLQPLDFKE NPEQSRVTIN NWVANKTEGR IKDVIPQGAI DELTALVLVN TIYFKGLWKS KFSPENTRKE PFHKVDGQSC LVPMMYQEGK FKYRRVGEGT QVLEMPFKGD DITMVLILPK PEKSLAKVEQ ELTPELLQEW LDELSEVMLV VHVPRFRIED SFSLKEQLQD MGLVDLFSPE KSQLPGIIAE GRDDLFVSDA FHKAFLEVNE
3D Structure
ModBase 3D Structure for Q5M7T5
Specificity
The antibody is a rabbit polyclonal antibody raised against AT. It has beenselected for its ability to recognize AT in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Concentration
200ug/ml (lot specific)
Fragment
Antithrombin (Asp150~Arg426)
Organism Species
Rattus norvegicus (Rat)
Immunogen
Recombinant Antithrombin (Asp150~Arg426) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2064845
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.
Other Notes
Small volumes of anti-AT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-AT antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-AT antibody
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Western Blot (WB) of anti-AT antibody
Western Blot: Sample: Rat Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat AT Antibody Second Ab: 0.2ug/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Western Blot (WB) of anti-AT antibody
Western Blot: Sample: Recombinant protein.
Immunohistochemistry (IHC) of anti-AT antibody
DAB staining on IHC-P. Samples: Rat Tissue)
NCBI/Uniprot data below describe general gene information for AT. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001012027.1
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NCBI GenBank Nucleotide #
NM_001012027.1
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UniProt Primary Accession #
Q5M7T5
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Molecular Weight
52,234 Da
NCBI Official Full Name
antithrombin-III
NCBI Official Synonym Full Names
serpin family C member 1
NCBI Official Symbol
Serpinc1??[Similar Products]
NCBI Protein Information
antithrombin-III
UniProt Protein Name
Serine (Or cysteine) peptidase inhibitor, clade C (Antithrombin), member 1
UniProt Synonym Protein Names
Serine (Or cysteine) peptidase inhibitor, clade C (Antithrombin), member 1
Protein Family
Antithrombin
UniProt Gene Name
Serpinc1??[Similar Products]
Research Articles on AT
1. SerpinC1 insufficiency in increasing the severity of renal ischemia/reperfusion injury.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
