Product Name
Antithrombin (AT), Recombinant Protein
Full Product Name
Recombinant Antithrombin (AT)
Product Gene Name
AT recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHH H SSGLVPRGSH MASMTGGQQM GRGSEF- D QIHFFFAKLN CRLYRKANKS SNLVSANRLF GDKSLTFNES YQDVSEIVYG AKLQPLDFKE NPEQSRVTIN NWVANKTEGR IKDVIPQGAI DELTALVLVN TIYFKGLWKS KFSPENTRKE PFHKVDGQSC LVPMMYQEGK FKYRRVGEGT QVLEMPFKGD DITMVLILPK PEKSLAKVEQ ELTPELLQEW LDELSEVMLV VHVPRFRIED SFSLKEQLQD MGLVDLFSPE KSQLPGIIAE GRDDLFVSDA FHKAFLEVNE EGSEAAASTS VVITGR
3D Structure
ModBase 3D Structure for P01008
Host
Host: E Coli
Source: Prokaryotic expression
Form/Format
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Concentration
5.9 (lot specific)
Predicted Molecular Mass
35.3kDa
Accurate Molecular Mass (KD)
35kDa
Endotoxin
<1.0EU per 1ug (determined by the LAL method)
Expression System
Prokaryotic expression
Tag
two N-terminal Tags, His-tag and T7-tag
Organism Species
Rattus norvegicus (Rat)
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.
Other Notes
Small volumes of AT recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for AT recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
SDS-Page of AT recombinant protein
NCBI/Uniprot data below describe general gene information for AT. It may not necessarily be applicable to this product.
NCBI Accession #
CAA48690.1
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UniProt Primary Accession #
P01008
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UniProt Secondary Accession #
P78439; P78447; Q13815; Q5TC78; Q7KZ43; Q7KZ97; Q9UC78; B2R6P0[Other Products]
UniProt Related Accession #
P01008[Other Products]
Molecular Weight
52,602 Da
NCBI Official Full Name
antithrombin
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade C (antithrombin), member 1
NCBI Official Symbol
SERPINC1??[Similar Products]
NCBI Official Synonym Symbols
AT3; AT3D; ATIII; THPH7
??[Similar Products]
NCBI Protein Information
antithrombin-III
UniProt Protein Name
Antithrombin-III
UniProt Synonym Protein Names
Serpin C1
Protein Family
Antithrombin
UniProt Gene Name
SERPINC1??[Similar Products]
UniProt Synonym Gene Names
AT3; ATIII??[Similar Products]
UniProt Entry Name
ANT3_HUMAN
NCBI Summary for AT
The protein encoded by this gene is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. The protein includes two functional domains: the heparin binding-domain at the N-terminus of the mature protein, and the reactive site domain at the C-terminus. The inhibitory activity is enhanced by the presence of heparin. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency. [provided by RefSeq, Jul 2009]
UniProt Comments for AT
SERPINC1: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D). AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin- binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations. Belongs to the serpin family.
Protein type: Secreted, signal peptide; Secreted; Inhibitor
Chromosomal Location of Human Ortholog: 1q25.1
Cellular Component: extracellular space; plasma membrane; extracellular region
Molecular Function: heparin binding; serine-type endopeptidase inhibitor activity; protein binding; protease binding
Biological Process: negative regulation of inflammatory response; blood coagulation; response to nutrient
Disease: Antithrombin Iii Deficiency
Research Articles on AT
1. A (p.A391T), in the AT gene, which reduces its heparin binding capacity and might be associated with resistance to heparin">We identified a novel hereditary mutation, g.1267G>A (p.A391T), in the AT gene, which reduces its heparin binding capacity and might be associated with resistance to heparin
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
