Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; FOXO1; FKHR; FOXO1A

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This FKHR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 454-482 amino acids from the Central region of human FKHR.

Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Vial Concentration: 2 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-FOXO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

FKHR belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain.

Cancer; Cell Biology; Metabolism; Neuroscience; Signal Transduction

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)

WB~~1:1000

Western blot analysis of FKHR antibody (Center D469) in 293 cell line lysates (35ug/lane). FKHR (arrow) was detected using the purified Pab.

(LEFT)Formalin-fixed and paraffin-embedded human brain tissue reacted with FKHR Antibody (Center D469), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
(RIGHT)Flow cytometric analysis of 293 cells using FKHR Antibody (Center D469)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

69662

forkhead box O1

forkhead box protein O1

Forkhead box protein O1

FKHR; FOXO1A??[Similar Products]

FOXO1_HUMAN

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008]

FOXO1A: a transcription factor of the forkhead family. A central regulator of metabolism in several cell types. May play an important role in myogenic growth and differentiation, and in the regulation of lipolysis in adipocytes by controlling the expression of adipose triglyceride lipase (ATGL), the rate-limiting lipolytic enzyme. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. Contains 1 fork-head domain.

Protein type: Nuclear receptor co-regulator; Transcription factor; Oncoprotein; DNA-binding

Chromosomal Location of Human Ortholog: 13q14.1

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; protein phosphatase 2A binding; sequence-specific DNA binding; ubiquitin protein ligase binding; chromatin binding

Biological Process: fat cell differentiation; transcription from RNA polymerase II promoter; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; apoptosis; positive regulation of transcription, DNA-dependent; thermoregulation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of gluconeogenesis; positive regulation of autophagy; epidermal growth factor receptor signaling pathway; blood vessel development; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; negative regulation of fat cell differentiation; negative regulation of stress-activated MAPK cascade; cellular response to starvation; endocrine pancreas development; protein amino acid acetylation; cellular response to insulin stimulus; insulin receptor signaling pathway; positive regulation of protein catabolic process; innate immune response; cell glucose homeostasis; autophagy; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; response to DNA damage stimulus; negative regulation of apoptosis

Disease: Rhabdomyosarcoma 2

Jung,Y.A., Biochem. Biophys. Res. Commun. 386 (4), 757-761 (2009)
Lau,C.J., Cancer Res. 69 (13), 5433-5440 (2009)

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