Anti -FKHR, phosphorylated (Ser256) (Forkhead Family Transcription Factor)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 13; NC_000013.10 (41129801..41240734, complement). Location: 13q14.1

Polyclonal

IgG

Rabbit

Detects endogenous levels of total levels of FKHR only when phosphorylated at Ser256. Crossreacts with phosphorylated AFX. Species Crossreactivity: Human, mouse, rat.

Affinity Purified
Purified by Protein A affinity chromatography.

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-FOXO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The Forkhead family of transcription factors is involved in tumorigenesis in rhabdomyosarcoma and acute leukemias. Within the family, three members (FKHR, AFX and FKHRL1) have sequence similarity to the nematode homologue DAF-16, recently shown to mediate signaling via a pathway involving IGFR1, PI3K and Akt. There are three Akt phosphorylation sites in the FKHR proteins: Thr24, Ser256 and Ser319. Phosphorylation of FKHR family members at these sites by Akt promotes cell survival and regulates the cell cycle. Phosphorylation regulates FKHR nuclear translocation and target gene transcription.

Antibodies; Abs to Transcription Factors

ELISA (EL/EIA), Western Blot (WB)

Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween 20 at 4 degree C with gentle shaking overnight.

~75kD[Similar Products]

forkhead box O1

forkhead box protein O1; forkhead box protein O1A; forkhead, Drosophila, homolog of, in rhabdomyosarcoma

Forkhead box protein O1

FKHR; FOXO1A??[Similar Products]

FOXO1_HUMAN

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008]

FOXO1A: a transcription factor of the forkhead family. A central regulator of metabolism in several cell types. May play an important role in myogenic growth and differentiation, and in the regulation of lipolysis in adipocytes by controlling the expression of adipose triglyceride lipase (ATGL), the rate-limiting lipolytic enzyme. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. Contains 1 fork-head domain.

Protein type: Oncoprotein; Transcription factor; DNA-binding; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 13q14.1

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; protein phosphatase 2A binding; sequence-specific DNA binding; ubiquitin protein ligase binding; chromatin binding

Biological Process: transcription from RNA polymerase II promoter; fat cell differentiation; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; apoptosis; positive regulation of transcription, DNA-dependent; thermoregulation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of gluconeogenesis; positive regulation of autophagy; epidermal growth factor receptor signaling pathway; blood vessel development; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; negative regulation of fat cell differentiation; cellular response to starvation; negative regulation of stress-activated MAPK cascade; endocrine pancreas development; protein amino acid acetylation; cellular response to insulin stimulus; insulin receptor signaling pathway; positive regulation of protein catabolic process; autophagy; cell glucose homeostasis; innate immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; response to DNA damage stimulus; negative regulation of apoptosis

Disease: Rhabdomyosarcoma 2

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