Anti -FKHR, phosphorylated (Ser329) (Forkhead Family Transcription Factor)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 13; NC_000013.10 (41129801..41240734, complement). Location: 13q14.1

Polyclonal

IgG

Rabbit

Recognizes human GST-FKHR phosphorylated at Ser329, Mr 105kD. Species Crossreactivity: mouse and rat.

Affinity Purified
Purified by immunoaffinity chromatography.

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-FOXO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Antibodies; Abs to Transcription Factors

Western Blot (WB)

Suitable for use in Western Blot.
Dilution: Western Blot: 1:3000-1:10,000. Detected 400ng of F4523-15 phosphorylated with 0.5ug A1125-06. 400ng of F4523-15 and FKHR fusion protein incubated with 500ng Akt1/PKBa were resolved by electrophoresis, transferred to nitrocellulose and probed with F4523-21(1:3000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Note: This antibody is unlikely to detect endogenous phospho-FKHR in cell lysates via straight Westrern Blot analysis.

69,662 Da[Similar Products]

forkhead box O1

forkhead box protein O1; forkhead box protein O1A; forkhead, Drosophila, homolog of, in rhabdomyosarcoma

Forkhead box protein O1

FKHR; FOXO1A??[Similar Products]

FOXO1_HUMAN

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008]

FOXO1A: a transcription factor of the forkhead family. A central regulator of metabolism in several cell types. May play an important role in myogenic growth and differentiation, and in the regulation of lipolysis in adipocytes by controlling the expression of adipose triglyceride lipase (ATGL), the rate-limiting lipolytic enzyme. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. Contains 1 fork-head domain.

Protein type: DNA-binding; Nuclear receptor co-regulator; Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 13q14.1

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; protein phosphatase 2A binding; sequence-specific DNA binding; ubiquitin protein ligase binding; chromatin binding

Biological Process: fat cell differentiation; transcription from RNA polymerase II promoter; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; apoptosis; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; thermoregulation; positive regulation of gluconeogenesis; positive regulation of autophagy; epidermal growth factor receptor signaling pathway; blood vessel development; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; cellular response to starvation; negative regulation of fat cell differentiation; negative regulation of stress-activated MAPK cascade; endocrine pancreas development; protein amino acid acetylation; cellular response to insulin stimulus; positive regulation of protein catabolic process; insulin receptor signaling pathway; innate immune response; autophagy; cell glucose homeostasis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; response to DNA damage stimulus; negative regulation of apoptosis

Disease: Rhabdomyosarcoma 2

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