Product Name
alpha Tubulin (TUBA1B), Polyclonal Antibody
Full Product Name
alpha Tubulin Antibody
Product Synonym Names
Tubulin alpha-1B chain; Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain; TUBA1B
Product Gene Name
anti-TUBA1B antibody
[Similar Products]
Antibody/Peptide Pairs
alpha Tubulin peptide (MBS9230709) is used for blocking the activity of alpha Tubulin antibody (MBS9217273)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P68363
Species Reactivity
Human; Predicted Reactivity: Bovine, Chicken, Human, Mouse, Pig, Rat
Specificity
This alpha Tubulin antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 408-439 amino acids from human alpha Tubulin.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Subtitle
Purified Rabbit Polyclonal Antibody (Pab)
Cellular Location
Cytoplasm, cytoskeleton.
Antigen Type
Synthesized Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-TUBA1B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TUBA1B antibody
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Product Categories/Family for anti-TUBA1B antibody
New Antibodies; Primary Antibodies; Neuroscience; Signal Transduction
Applications Tested/Suitable for anti-TUBA1B antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-TUBA1B antibody
WB:~~1:2000
Western Blot (WB) of anti-TUBA1B antibody
All lanes : Anti-alpha Tubulin Antibody at 1:2000 dilution
Lane 1: 293T/17 whole cell lysate
Lane 2: A431 whole cell lysate
Lane 3: Hela whole cell lysate
Lane 4: HepG2 whole cell lysate
Lane 5: Jurkat whole cell lysate
Lane 6: Raji whole cell lysate
Lysates/proteins at 20 ug per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 50 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
NCBI/Uniprot data below describe general gene information for TUBA1B. It may not necessarily be applicable to this product.
NCBI Accession #
NP_006073.2
[Other Products]
NCBI GenBank Nucleotide #
NM_006082.2
[Other Products]
UniProt Primary Accession #
P68363
[Other Products]
UniProt Secondary Accession #
P04687; P05209; Q27I68; Q8WU19[Other Products]
UniProt Related Accession #
P68363[Other Products]
Molecular Weight
50152 Da
NCBI Official Full Name
tubulin alpha-1B chain
NCBI Official Synonym Full Names
tubulin alpha 1b
NCBI Official Symbol
TUBA1B??[Similar Products]
NCBI Official Synonym Symbols
K-ALPHA-1
??[Similar Products]
NCBI Protein Information
tubulin alpha-1B chain
UniProt Protein Name
Tubulin alpha-1B chain
UniProt Synonym Protein Names
Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain
UniProt Gene Name
TUBA1B??[Similar Products]
UniProt Entry Name
TBA1B_HUMAN
UniProt Comments for TUBA1B
TUBA1B: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Belongs to the tubulin family.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: 12q13.12
Cellular Component: cytoplasmic microtubule; microtubule; myelin sheath
Molecular Function: double-stranded RNA binding; GTP binding; GTPase activity; protein binding; structural constituent of cytoskeleton; structural molecule activity; ubiquitin protein ligase binding
Biological Process: cell division; cytoskeleton-dependent intracellular transport; metabolic process; microtubule cytoskeleton organization and biogenesis; microtubule-based process
Product References and Citations for anti-TUBA1B antibody
Cowan N.J.,et al.Mol. Cell. Biol. 3:1738-1745 (1983). de Hostos E.L.,et al.Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases. Li Q.,et al.Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases. Lubec G.,et al.Submitted (DEC-2008) to UniProtKB. Bienvenut W.V.,et al.Submitted (MAR-2009) to UniProtKB.
Research Articles on TUBA1B
1. Results suggest that lithium chloride (LiCl) treatments activate alpha-tubulin N-acetyltransferase 1 (alphaTAT1) by the inhibition of glycogen synthase kinase 3 beta (GSK-3beta) and promote the alpha-tubulin acetylation, and then elongate the primary cilia.
Precautions
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Disclaimer
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