Product Name
alpha Tubulin (TUBA1B), Monoclonal Antibody
Full Product Name
Anti-Phospho-alpha Tubulin (Y272) Rabbit Monoclonal Antibody
Product Synonym Names
Tubulin alpha-1B chain; Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain; TUBA1B
Product Gene Name
anti-TUBA1B antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P68363
Purity/Purification
Affinity-chromatography
Immunogen
A synthesized peptide derived from human Phospho-alpha Tubulin (Y272)
Subcellular Localization
Cytoplasm, cytoskeleton.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-TUBA1B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TUBA1B antibody
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Product Categories/Family for anti-TUBA1B antibody
Signal Transduction; Cytoskeleton/ECM; Cytoskeleton; microtubules; tubulin
Applications Tested/Suitable for anti-TUBA1B antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-TUBA1B antibody
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
Western Blot (WB) of anti-TUBA1B antibody
Western blot analysis on Jurkat cell lysates treated with pervanadate using alpha Tubulin (phospho Y272) Antibody (MBS1750289).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUBA1B monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TUBA1B
NCBI/Uniprot data below describe general gene information for TUBA1B. It may not necessarily be applicable to this product.
NCBI Accession #
NP_006073.2
[Other Products]
NCBI GenBank Nucleotide #
NM_006082.2
[Other Products]
UniProt Primary Accession #
P68363
[Other Products]
UniProt Secondary Accession #
P04687; P05209; Q27I68; Q8WU19[Other Products]
UniProt Related Accession #
P68363[Other Products]
Molecular Weight
50152 MW
NCBI Official Full Name
tubulin alpha-1B chain
NCBI Official Synonym Full Names
tubulin alpha 1b
NCBI Official Symbol
TUBA1B??[Similar Products]
NCBI Official Synonym Symbols
K-ALPHA-1
??[Similar Products]
NCBI Protein Information
tubulin alpha-1B chain
UniProt Protein Name
Tubulin alpha-1B chain
UniProt Synonym Protein Names
Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain
UniProt Gene Name
TUBA1B??[Similar Products]
UniProt Comments for TUBA1B
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Research Articles on TUBA1B
1. Data show that retinal endothelial cells (HREC) treated with low molecular weight fraction of commercial 5% human serum albumin (LMWF5A) exhibit a rapid increase in the amount and distribution of acetylated alpha-tubulin.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
