Full Product Name
AOS1 Antibody
Product Gene Name
anti-AOS1 antibody
[Similar Products]
Product Synonym Gene Name
SAE1[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9UBE0
Species Reactivity
Human, Mouse, Rat
Specificity
AOS1 Antibody detects endogenous levels of total AOS1 protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (lot specific)
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human AOS1.
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-AOS1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-AOS1 antibody
Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-AOS1 antibody
WB: 1:500~1:1000
IF: 1:100~1:500
ELISA: 1:20000
Immunofluorescence (IF) of anti-AOS1 antibody
Immunofluorescence analysis of HUVEC cells, using AOS1 Antibody. The picture on the right is blocked with the synthesized peptide.
Western Blot (WB) of anti-AOS1 antibody
Western blot analysis of lysates from 293 cells, using AOS1 Antibody. The lane on the right is blocked with the synthesized peptide.
NCBI/Uniprot data below describe general gene information for AOS1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001139185.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001145713.1
[Other Products]
UniProt Primary Accession #
Q9UBE0
[Other Products]
UniProt Secondary Accession #
O95717; Q9P020; B2RDP5; B3KMQ2; F5GXX7; G3XAK6[Other Products]
UniProt Related Accession #
Q9UBE0[Other Products]
NCBI Official Full Name
SUMO-activating enzyme subunit 1 isoform b
NCBI Official Synonym Full Names
SUMO1 activating enzyme subunit 1
NCBI Official Symbol
SAE1??[Similar Products]
NCBI Official Synonym Symbols
AOS1; SUA1; UBLE1A; HSPC140
??[Similar Products]
NCBI Protein Information
SUMO-activating enzyme subunit 1
UniProt Protein Name
SUMO-activating enzyme subunit 1
UniProt Synonym Protein Names
Ubiquitin-like 1-activating enzyme E1A
Protein Family
DNA damage tolerance protein
UniProt Gene Name
SAE1??[Similar Products]
UniProt Synonym Gene Names
AOS1; SUA1; UBLE1A??[Similar Products]
NCBI Summary for AOS1
Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]
UniProt Comments for AOS1
The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Research Articles on AOS1
1. the expression of SENP8, SAE1, PIAS1, PIAS2 and ZMIZ1 is deregulated in the majority of PTC tissues, likely contributing to the PTC phenotype.
Precautions
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Disclaimer
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