Homeobox protein EMX2; Empty spiracles homolog 2; Empty spiracles-like protein 2; EMX2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This EMX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-138 amino acids from the Central region of human EMX2.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.5 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-EMX2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This gene encodes a homeobox-containing transcription
factor that is the homolog to the 'empty spiracles' gene in
Drosophila. Research on this gene in humans has focused on its
expression in three tissues: dorsal telencephalon, olfactory
neuroepithelium, and urogenetial system. It is expressed in the
dorsal telencephalon during development in a low rostral-lateral to
high caudal-medial gradient and is proposed to pattern the
neocortex into defined functional areas. It is also expressed in
embryonic and ***** olfactory neuroepithelia where it complexes
with eukaryotic translation initiation factor 4E (eIF4E) and
possibly regulates mRNA transport or translation. In the developing
urogenital system, it is expressed in epithelial tissues and is
negatively regulated by HOXA10. Alternative splicing results in
multiple transcript variants encoding distinct proteins.

Neuroscience

Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)

WB~~1:1000

EMX2 Antibody (Center) western blot analysis in HL-60 cell line lysates (15ug/lane).This demonstrates the EMX2 antibody detected the EMX2 protein (arrow).

EMX2 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

28303

empty spiracles homeobox 2

homeobox protein EMX2

Homeobox protein EMX2

EMX2_HUMAN

This gene encodes a homeobox-containing transcription factor that is the homolog to the 'empty spiracles' gene in Drosophila. Research on this gene in humans has focused on its expression in three tissues: dorsal telencephalon, olfactory neuroepithelium, and urogenetial system. It is expressed in the dorsal telencephalon during development in a low rostral-lateral to high caudal-medial gradient and is proposed to pattern the neocortex into defined functional areas. It is also expressed in embryonic and ***** olfactory neuroepithelia where it complexes with eukaryotic translation initiation factor 4E (eIF4E) and possibly regulates mRNA transport or translation. In the developing urogenital system, it is expressed in epithelial tissues and is negatively regulated by HOXA10. Alternative splicing results in multiple transcript variants encoding distinct proteins.[provided by RefSeq, Sep 2009]

EMX2: Transcription factor, which in cooperation with EMX2, acts to generate the boundary between the roof and archipallium in the developing brain. May function in combinations with OTX1/2 to specify cell fates in the developing central nervous system. Defects in EMX2 are the cause of schizencephaly (SCHZC). Schizencephaly is an extremely rare human congenital disorder characterized by a full-thickness cleft within the cerebral hemispheres. These clefts are lined with gray matter and most commonly involve the parasylvian regions. Large portions of the cerebral hemispheres may be absent and replaced by cerebro- spinal fluid. Belongs to the EMX homeobox family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 10q26.1

Cellular Component: nucleus

Molecular Function: protein binding; sequence-specific DNA binding

Biological Process: anterior/posterior pattern formation; response to drug; forebrain cell migration; neuron differentiation; dentate gyrus development; regulation of transcription, DNA-dependent; cell proliferation in forebrain; cerebral cortex regionalization

Disease: Schizencephaly

Inkster, B., et al. Neuroimage (2010) In press :
Bayatti, N., et al. Eur. J. Neurosci. 28(8):1449-1456(2008)
Merello, E., et al. Am. J. Med. Genet. A 146A (9), 1142-1150 (2008) :
Treloar, S.A., et al. Mol. Hum. Reprod. 13(8):587-594(2007)

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