Full Product Name
Anti-SIX3 Antibody (Internal) IHC-plus
Product Synonym Names
Rabbit Polyclonal (IgG) to Mouse SIX3; Mouse SIX3; SIX3; HPE2; Sine oculis homeobox homolog 3; SIX homeobox 3; Homeobox protein SIX3
Product Gene Name
anti-SIX3 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O95343
Species Reactivity
Mouse, Human
Specificity
This antibody reacts with mouse Six3. Cross-reactivity with Six3 from other sources has not been determined.
Purity/Purification
Immunoaffinity purified
Form/Format
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Concentration
1 mg/ml (lot specific)
Immunogen
SIX3 antibody was raised against this affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Six3 protein.
Immunogen Description
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Six3 protein.
Immunogen Type
Synthetic peptide
Antigen Modification
Internal
Preparation and Storage
Short term 4 degree C
Long term aliquot and store at -20 degree C
Avoid freeze thaw cycles. Store undiluted.
Other Notes
Small volumes of anti-SIX3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SIX3 antibody
SIX3 Antibody, HPE2 Antibody, Sine oculis homeobox homolog 3 Antibody, SIX homeobox 3 Antibody, Homeobox protein SIX3 Antibody Description: Transcriptional regulator which can act as both a transcriptional repressor and activator by binding a ATTA homeodomain core recognition sequence on these target genes. During forebrain development represses WNT1 expression allowing zona limitans intrathalamica formation and thereby ensuring proper anterio-posterior patterning of the diencephalon and formation of the rostral diencephalon.
Applications Tested/Suitable for anti-SIX3 antibody
Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-SIX3 antibody
ELISA (1:10000 - 1:15000)
IF (1:200 - 1:1000)
IHC-P (5 ug/ml)
WB (1:500 - 1:2000)
Immunohistochemistry (IHC) of anti-SIX3 antibody
Anti-SIX3 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunohistochemistry (IHC) of anti-SIX3 antibody
Immunohistochemistry of Rabbit anti-Six-3 antibody. Tissue: retina and brain basal ganglia (LGE) Fixation: PFA fixed and embedded in OCT/Tissue Tek for cryo Antigen retrieval: not required Primary antibody: SIX3 antibody at 1:500 for overnight at RT Secondary antibody: Donkey anti Rabbit Cy3 secondary antibody Localization: nuclear Staining: antibody visualized as red signal
NCBI/Uniprot data below describe general gene information for SIX3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_005404.1
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NCBI GenBank Nucleotide #
NM_005413.3
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UniProt Primary Accession #
O95343
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UniProt Secondary Accession #
Q53T42; D6W5A5[Other Products]
UniProt Related Accession #
O95343[Other Products]
Molecular Weight
35,487 Da
NCBI Official Full Name
homeobox protein SIX3
NCBI Official Synonym Full Names
SIX homeobox 3
NCBI Official Symbol
SIX3??[Similar Products]
NCBI Official Synonym Symbols
HPE2
??[Similar Products]
NCBI Protein Information
homeobox protein SIX3
UniProt Protein Name
Homeobox protein SIX3
UniProt Synonym Protein Names
Sine oculis homeobox homolog 3
Protein Family
Homeobox protein
UniProt Gene Name
SIX3??[Similar Products]
UniProt Entry Name
SIX3_HUMAN
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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