Full Product Name
SIX3 siRNA (Mouse)
Product Synonym Names
Homeobox protein SIX3; Sine oculis homeobox homolog 3
Product Gene Name
SIX3 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q62233
Specificity
SIX3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SIX3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of SIX3 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
SIX3 sirna
siRNA to inhibit SIX3 expression using RNA interference
Applications Tested/Suitable for SIX3 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for SIX3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_035511.2
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NCBI GenBank Nucleotide #
NM_011381.4
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UniProt Primary Accession #
Q62233
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UniProt Secondary Accession #
P70176; P70177; Q4QQQ3[Other Products]
UniProt Related Accession #
Q62233[Other Products]
Molecular Weight
30,610 Da
NCBI Official Full Name
homeobox protein SIX3
NCBI Official Synonym Full Names
sine oculis-related homeobox 3
NCBI Official Symbol
Six3??[Similar Products]
NCBI Official Synonym Symbols
Six3a; Six3b; Six3beta; Six3alpha; E130112M24Rik
??[Similar Products]
NCBI Protein Information
homeobox protein SIX3
UniProt Protein Name
Homeobox protein SIX3
UniProt Synonym Protein Names
Sine oculis homeobox homolog 3
Protein Family
Homeobox protein
UniProt Gene Name
Six3??[Similar Products]
UniProt Entry Name
SIX3_MOUSE
UniProt Comments for SIX3
SIX3: May be involved in visual system development. Defects in SIX3 are the cause of holoprosencephaly type 2 (HPE2). Holoprosencephaly (HPE) is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability. Belongs to the SIX/Sine oculis homeobox family.
Protein type: Nuclear receptor co-regulator
Cellular Component: nucleus
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; DNA binding; sequence-specific DNA binding; histone deacetylase binding; transcription coactivator activity; transcription factor activity; receptor binding
Biological Process: lens development in camera-type eye; transcription from RNA polymerase II promoter; negative regulation of Wnt receptor signaling pathway; cell proliferation in forebrain; multicellular organismal development; forebrain dorsal/ventral pattern formation; telencephalon regionalization; diencephalon development; regulation of transcription, DNA-dependent; protein import into nucleus; camera-type eye development; transcription, DNA-dependent; epithelial cell maturation; proximal/distal axis specification; neuroblast differentiation; circadian behavior; regulation of cell proliferation; negative regulation of neuron differentiation; telencephalon development; eye development; protein import into nucleus, translocation; pituitary gland development; forebrain anterior/posterior pattern formation; positive regulation of transcription from RNA polymerase II promoter; brain development; negative regulation of transcription, DNA-dependent
Research Articles on SIX3
1. Sox2 directly activates the Six3 forebrain enhancer.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
