Product Name
Homeobox protein SIX3 (six3), Recombinant Protein
Full Product Name
Recombinant Oryzias latipes Homeobox protein SIX3 (six3)
Product Gene Name
six3 recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
1-272aa, full length protein
Sequence
MVFRAPLDFI SASRLLLPHF ADAPPVLSRS RSPEHPPAGF LSLALPGLCF SATQIASVCE TLEETGDIER LARFLWSLPV NTDGRDSISE HESVQRARAV VAFHTGCYRE LYRILETHRF TRASHSKLQA MWLEAHYREA EKLRGRPLGP VDKYRVRKKF PLPRTIWDGE QKTHCFKERT RGLLREWYLQ DPYPNPGKKR ELAHATGLTP TQVGNWFKNR RQRDRAAAAK NRLQHHRICP DSACTLSGGD SSERADGDTF LSVTDSDSDL DV
3D Structure
ModBase 3D Structure for O73916
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
>85% (SDS-PAGE) (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Species
Oryzias latipes (Medaka fish) (Japanese ricefish)
Storage Buffer
Tris-based buffer, 50% glycerol
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of six3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
NCBI/Uniprot data below describe general gene information for six3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001098230.1
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NCBI GenBank Nucleotide #
NM_001104760.1
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UniProt Primary Accession #
O73916
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UniProt Related Accession #
O73916[Other Products]
Molecular Weight
30,946 Da
NCBI Official Full Name
homeobox protein SIX3
NCBI Official Symbol
six3??[Similar Products]
NCBI Protein Information
homeobox protein SIX3
UniProt Protein Name
Homeobox protein SIX3
UniProt Synonym Protein Names
Sine oculis homeobox homolog 3
Protein Family
Homeobox protein
UniProt Gene Name
six3??[Similar Products]
UniProt Comments for six3
Transcriptional regulator which can act as both a transcriptional repressor and activator by binding a ATTA homeodomain core recognition sequence on these target genes. During forebrain development represses WNT1 expression allowing zona limitans intrathalamica formation and thereby ensuring proper anterio-posterior patterning of the diencephalon and formation of the rostral diencephalon. Acts as a direct upstream activator of SHH expression in the rostral diencephalon ventral midline and that in turn SHH maintains its expression. In addition, Six3 activity is required for the formation of the telencephalon. During postnatal stages of brain development is necessary for ependymal cell maturation by promoting the maturation of radial glia into ependymal cells through regulation of neuroblast proliferation and migration. Acts on the proliferation and differentiation of neural progenitor cells through activating transcription of CCND1 AND CCND2. During early lens formation plays a role in lens induction and specification by activating directly PAX6 in the presumptive lens ectoderm. In turn PAX6 activates SIX3 resulting in activation of PDGFRA and CCND1 promoting cell proliferation. Also is required for the neuroretina development by directly suppressing WNT8B expression in the anterior neural plate territory. Its action during retina development and lens morphogenesis is AES and TLE4-dependent manner. Furthermore, during eye development regulates several genes expression. Before and during early lens development represses the CRYGF promoter by binding a SIX repressor element. Directly activates RHO transcription, or cooperates with CRX or NRL. Six3 functions also in the formation of the proximodistal axis of the optic cup, and promotes the formation of optic vesicles-like structures. During pituitary development, acts in parallel or alternatively with HESX1 to control cell proliferation through Wnt/beta-catenin pathway. Plays a role in eye development by suppressing WNT1 expression and in dorsal-ventral patterning by repressing BMP signaling pathway.
Precautions
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Disclaimer
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