Zinc finger protein GLIS2; GLI-similar 2; Neuronal Krueppel-like protein; GLIS2; NKL

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This GLIS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 173-202 amino acids from the Central region of human GLIS2.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.5 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-GLIS2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This gene is a member of the GLI-similar zinc finger
protein family and encodes a nuclear transcription factor with five
C2H2-type zinc finger domains. The protein encoded by this gene is
widely expressed at low levels in the neural tube and peripheral
nervous system and likely promotes neuronal differentiation. It is
abundantly expressed in the kidney and may have a role in the
regulation of kidney morphogenesis. p120 regulates the expression
level of this protein and induces the cleavage of this protein's
C-terminal zinc finger domain. This protein also promotes the
nuclear translocation of p120. Mutations in this gene cause
nephronophthisis (NPHP), an autosomal recessive kidney disease
characterized by tubular basement membrane disruption, interstitial
lymphohistiocytic cell infiltration, and development of cysts at
the corticomedullary border of the kidneys.

Neuroscience; Signal Transduction

Western Blot (WB), ELISA (EIA)

WB~~1:1000

GLIS2 Antibody (Center) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the GLIS2 antibody detected the GLIS2 protein (arrow).

55689

GLIS family zinc finger 2

zinc finger protein GLIS2

Zinc finger protein GLIS2

NKL??[Similar Products]

GLIS2_HUMAN

This gene is a member of the GLI-similar zinc finger protein family and encodes a nuclear transcription factor with five C2H2-type zinc finger domains. The protein encoded by this gene is widely expressed at low levels in the neural tube and peripheral nervous system and likely promotes neuronal differentiation. It is abundantly expressed in the kidney and may have a role in the regulation of kidney morphogenesis. p120 regulates the expression level of this protein and induces the cleavage of this protein's C-terminal zinc finger domain. This protein also promotes the nuclear translocation of p120. Mutations in this gene cause nephronophthisis (NPHP), an autosomal recessive kidney disease characterized by tubular basement membrane disruption, interstitial lymphohistiocytic cell infiltration, and development of cysts at the corticomedullary border of the kidneys.[provided by RefSeq, Jan 2010]

GLIS2: Can act either as a transcriptional repressor or as a transcriptional activator, depending on the cell context. Acts as a repressor of the Hedgehog signaling pathway. Represses the Hedgehog-dependent expression of Wnt4. Necessary to maintain the differentiated epithelial phenotype in renal cells through the inhibition of SNAI1, which itself induces the epithelial-to-mesenchymal transition. Represses transcriptional activation mediated by CTNNB1 in the Wnt signaling pathway. May act by recruiting the corepressors CTBP1 and HDAC3. May be involved in neuron differentiation. Defects in GLIS2 are the cause of nephronophthisis type 7 (NPHP7). NPHP7 is an autosomal recessive disorder resulting in end-stage renal disease during childhood or adolescence. It is a progressive tubulo-interstitial kidney disorder histologically characterized by modifications of the tubules with thickening of the basement membrane, interstitial fibrosis and, in the advanced stages, medullary cysts. Belongs to the GLI C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: cytoplasm; nuclear speck; nucleus

Molecular Function: protein binding; metal ion binding

Biological Process: nervous system development; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; negative regulation of transcription factor activity; negative regulation of smoothened signaling pathway; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; cell differentiation; negative regulation of transcription, DNA-dependent

Disease: Nephronophthisis 7

Attanasio, M., et al. Nat. Genet. 39(8):1018-1024(2007)
Hosking, C.R., et al. Mol. Biol. Cell 18(5):1918-1927(2007)
Kim, Y.S., et al. FEBS Lett. 581(5):858-864(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Kim, Y.S., et al. Nucleic Acids Res. 31(19):5513-5525(2003)

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