GLIS family zinc finger 2; GLI-similar 2; neuronal krueppel-like protein; NKL; NPHP7; GLIS family zinc finger 2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

GLIS2 antibody is human, mouse and rat reactive.

GLIS2 antibody is affinity chromatography purified via peptide column.

Liquid

1 mg/mL (lot specific)

GLIS2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

Small volumes of anti-GLIS2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

GLIS2, also known as neuronal Krueppel-like, is a 524 amino acid protein that belongs to the GLI C2H2-type zinc-finger protein family (1). GLIS2 can act either as a transcription repressor or as a transcription activator and may be involved in neuron differentiation (1,2). GLIS2 is expressed at high levels in kidney and at low levels in heart, lung and placenta. Mutations of GLIS2 have been suggested to be associated with development of progressive chronic kidney disease with characteristics resembling nephronophthisis (3,4).

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

GLIS2 antibody can be used for detection of GLIS2 by Western blot at 1 - 2 mug/ml. Antibody can also be used for immunohistochemistry starting at 5 mug/mL.

Western blot analysis of GLIS2 in K562 cell lysate with GLIS2 antibody at 1 μg/ml.

Immunohistochemistry of GLIS2 in mouse kidney tissue with GLIS2 antibody at 5 μg/ml.

Predicted: 58 kDa

Observed: 60 kDa

GLIS family zinc finger 2

zinc finger protein GLIS2; GLI-similar 2; nephrocystin-7; GLI-similar protein 2; neuronal Krueppel-like protein; Kruppel-like zinc finger protein GLIS2

Zinc finger protein GLIS2

NKL??[Similar Products]

GLIS2_HUMAN

This gene is a member of the GLI-similar zinc finger protein family and encodes a nuclear transcription factor with five C2H2-type zinc finger domains. The protein encoded by this gene is widely expressed at low levels in the neural tube and peripheral nervous system and likely promotes neuronal differentiation. It is abundantly expressed in the kidney and may have a role in the regulation of kidney morphogenesis. p120 regulates the expression level of this protein and induces the cleavage of this protein's C-terminal zinc finger domain. This protein also promotes the nuclear translocation of p120. Mutations in this gene cause nephronophthisis (NPHP), an autosomal recessive kidney disease characterized by tubular basement membrane disruption, interstitial lymphohistiocytic cell infiltration, and development of cysts at the corticomedullary border of the kidneys.[provided by RefSeq, Jan 2010]

GLIS2: Can act either as a transcriptional repressor or as a transcriptional activator, depending on the cell context. Acts as a repressor of the Hedgehog signaling pathway. Represses the Hedgehog-dependent expression of Wnt4. Necessary to maintain the differentiated epithelial phenotype in renal cells through the inhibition of SNAI1, which itself induces the epithelial-to-mesenchymal transition. Represses transcriptional activation mediated by CTNNB1 in the Wnt signaling pathway. May act by recruiting the corepressors CTBP1 and HDAC3. May be involved in neuron differentiation. Defects in GLIS2 are the cause of nephronophthisis type 7 (NPHP7). NPHP7 is an autosomal recessive disorder resulting in end-stage renal disease during childhood or adolescence. It is a progressive tubulo-interstitial kidney disorder histologically characterized by modifications of the tubules with thickening of the basement membrane, interstitial fibrosis and, in the advanced stages, medullary cysts. Belongs to the GLI C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: cytoplasm; nuclear speck; nucleus

Molecular Function: protein binding; metal ion binding

Biological Process: nervous system development; transcription, DNA-dependent; negative regulation of transcription factor activity; negative regulation of smoothened signaling pathway; positive regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; cell differentiation

Disease: Nephronophthisis 7

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.