Product Name
AP2S1, siRNA
Full Product Name
AP2S1 siRNA (Human)
Product Synonym Names
AP17; CLAPS2; AP-2 complex subunit sigma; Adapter-related protein complex 2 subunit sigma; Adaptor protein complex AP-2 subunit sigma; Clathrin assembly protein 2 sigma small chain; Clathrin coat assembly protein AP17; Clathrin coat-associated protein AP17; HA2 17 kDa subunit; Plasma membrane adaptor AP-2 17 kDa protein;
Sigma2-adaptin
Product Gene Name
AP2S1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P53680
Specificity
AP2S1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human AP2S1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of AP2S1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
AP2S1 sirna
siRNA to inhibit AP2S1 expression using RNA interference
Applications Tested/Suitable for AP2S1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for AP2S1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001288005.1
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NCBI GenBank Nucleotide #
NM_001301076.1
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UniProt Primary Accession #
P53680
[Other Products]
UniProt Secondary Accession #
O75977; Q6PK67; B2R4Z4[Other Products]
UniProt Related Accession #
P53680[Other Products]
Molecular Weight
12,417 Da
NCBI Official Full Name
AP-2 complex subunit sigma isoform 3
NCBI Official Synonym Full Names
adaptor-related protein complex 2, sigma 1 subunit
NCBI Official Symbol
AP2S1??[Similar Products]
NCBI Official Synonym Symbols
AP17; FBH3; HHC3; FBHOk; CLAPS2
??[Similar Products]
NCBI Protein Information
AP-2 complex subunit sigma
UniProt Protein Name
AP-2 complex subunit sigma
UniProt Synonym Protein Names
Adaptor protein complex AP-2 subunit sigma; Adaptor-related protein complex 2 subunit sigma; Clathrin assembly protein 2 sigma small chain; Clathrin coat assembly protein AP17; Clathrin coat-associated protein AP17; HA2 17 kDa subunit; Plasma membrane adaptor AP-2 17 kDa protein; Sigma2-adaptin
Protein Family
AP-2 complex
UniProt Gene Name
AP2S1??[Similar Products]
UniProt Synonym Gene Names
AP17; CLAPS2??[Similar Products]
UniProt Entry Name
AP2S1_HUMAN
NCBI Summary for AP2S1
One of two major clathrin-associated adaptor complexes, AP-2, is a heterotetramer which is associated with the plasma membrane. This complex is composed of two large chains, a medium chain, and a small chain. This gene encodes the small chain of this complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
UniProt Comments for AP2S1
AP2S1: Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein Transport via Transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin- coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif. Belongs to the adaptor complexes small subunit family. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 19q13.32
Cellular Component: plasma membrane; AP-2 adaptor complex; cytosol
Molecular Function: transporter activity; protein transporter activity
Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of epidermal growth factor receptor signaling pathway; synaptic transmission; intracellular protein transport; clathrin cage assembly; axon guidance; nerve growth factor receptor signaling pathway; viral reproduction; regulation of endocytosis; ephrin receptor signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class II; regulation of defense response to virus by virus
Disease: Hypocalciuric Hypercalcemia, Familial, Type Iii
Research Articles on AP2S1
1. The results affirm that a significant number of patients suspected of having Familial hypocalciuric hypercalcemia but proven negative for CASR mutation have AP2S1 p.R15 mutations.
Precautions
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Disclaimer
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