Rat Netrin-1 (NTN1) ELISA kit; NTN1L; Netrin-1; netrin 1; mouse; homolog of; netrin 1

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of rat NTN1. No significant cross-reactivity or interference between rat NTN1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of NTN1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS943237 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the netrin 1 (NTN1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NTN1. The ELISA analytical biochemical technique of the MBS943237 kit is based on NTN1 antibody-NTN1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NTN1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NTN1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NTN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NTN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NTN1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NTN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

67,873 Da

netrin 1

netrin-1

Netrin-1

NET1_RAT

plays a role in axon guidance during development; may also be involved in cell-cell interactions in the ***** nervous system [RGD, Feb 2006]

Function: Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in colorectal tumorigenesis by regulating apoptosis 1 PublicationManual assertion based on experiment in:Ref.2

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