Anti-MRE11A / MRE11 Antibody (aa68-706) IHC-plus; MRE11A; AT-like disease; Endo/exonuclease Mre11; HNGS1; MRE11; MRE11 homolog 1; MRE11 homolog A; MRE11B; ATLD; Mouse MRE11A; MRE11

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Recombinant protein corres-ponding to amino acids 68-706 of mouse Mre11 protein.

Immunoaffinity Purified

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.

1 mg/ml (lot specific)

+4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Small volumes of anti-MRE11A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)

ELISA (1:10000 - 1:50000), IF, IHC-P (5 ug/ml), WB (1:500 - 1:2000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Anti-MRE11A / MRE11 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Anti-Mre11 Antibody - Western Blot. Western blot of Affinity Purified anti-Mre11 antibody shows detection of a band ~80 kD corresponding to mouse Mre11 (arrowhead). Lanes 1-4 contain 0.5 ug, 0.3 ug, 0.1 ug and 0.05 ug of purified mouse Mre11 protein, respectively. After 4-20% SDS-PAGE and transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:1000 overnight at 4C. The membrane was then washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

81,070 Da

MRE11 meiotic recombination 11 homolog A (S. cerevisiae)

double-strand break repair protein MRE11A; AT-like disease; MRE11 homolog 1; MRE11 homolog A; endo/exonuclease Mre11; meiotic recombination 11 homolog 1; meiotic recombination 11 homolog A; DNA recombination and repair protein

Double-strand break repair protein MRE11A

HNGS1; MRE11; MRE11 homolog 1; MRE11 homolog A??[Similar Products]

MRE11_HUMAN

This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

MRE11A: a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. Alternative splicing results in two different isoforms.

Protein type: DNA repair, damage; Cell cycle regulation; Deoxyribonuclease; DNA-binding

Chromosomal Location of Human Ortholog: 11q21

Cellular Component: nucleoplasm; chromosome, telomeric region; Mre11 complex; chromatin; nucleus; cytosol

Molecular Function: protein C-terminus binding; ATP-dependent DNA helicase activity; protein binding; nuclease activity; DNA binding; single-stranded DNA specific endodeoxyribonuclease activity; endonuclease activity; manganese ion binding; double-stranded DNA binding; 3'-5' exonuclease activity; endodeoxyribonuclease activity

Biological Process: positive regulation of kinase activity; sister chromatid cohesion; negative regulation of DNA endoreduplication; telomere maintenance via telomerase; DNA repair; regulation of mitotic recombination; positive regulation of protein amino acid autophosphorylation; DNA catabolic process, endonucleolytic; double-strand break repair via nonhomologous end joining; double-strand break repair via homologous recombination; DNA duplex unwinding; DNA recombination; cell proliferation; base-excision repair; intra-S DNA damage checkpoint; nucleotide-excision repair; double-strand break repair; meiotic recombination; mitotic cell cycle G2/M transition DNA damage checkpoint; synapsis; positive regulation of interferon type I production; innate immune response; telomere maintenance; response to DNA damage stimulus

Disease: Ataxia-telangiectasia-like Disorder 1

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.