Product Name
MRE11A, siRNA
Full Product Name
MRE11A siRNA (Mouse)
Product Synonym Names
MRE11; Double-strand break repair protein MRE11A; MmMRE11A; Meiotic recombination 11 homolog 1; MRE11 homolog 1; Meiotic recombination 11 homolog A; MRE11 homolog A
Product Gene Name
MRE11A sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q61216
Specificity
MRE11A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MRE11A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of MRE11A sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
MRE11A sirna
siRNA to inhibit MRE11A expression using RNA interference
Applications Tested/Suitable for MRE11A sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for MRE11A. It may not necessarily be applicable to this product.
NCBI Accession #
NP_061206.1
[Other Products]
NCBI GenBank Nucleotide #
NM_018736.3
[Other Products]
UniProt Primary Accession #
Q61216
[Other Products]
UniProt Secondary Accession #
Q62430[Other Products]
UniProt Related Accession #
Q61216[Other Products]
Molecular Weight
77,094 Da
NCBI Official Full Name
double-strand break repair protein MRE11A isoform 1
NCBI Official Synonym Full Names
meiotic recombination 11 homolog A (S. cerevisiae)
NCBI Official Symbol
Mre11a??[Similar Products]
NCBI Official Synonym Symbols
Mre11; Mre11b
??[Similar Products]
NCBI Protein Information
double-strand break repair protein MRE11A
UniProt Protein Name
Double-strand break repair protein MRE11A
UniProt Synonym Protein Names
Meiotic recombination 11 homolog 1; MRE11 homolog 1; Meiotic recombination 11 homolog A; MRE11 homolog A
Protein Family
Double-strand break repair protein
UniProt Gene Name
Mre11a??[Similar Products]
UniProt Synonym Gene Names
Mre11; MmMRE11A; MRE11 homolog 1; MRE11 homolog A??[Similar Products]
UniProt Entry Name
MRE11_MOUSE
UniProt Comments for MRE11A
MRE11A: a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. Alternative splicing results in two different isoforms.
Protein type: DNA repair, damage; Deoxyribonuclease; DNA-binding; Cell cycle regulation
Cellular Component: nucleoplasm; chromosome, telomeric region; Mre11 complex; condensed nuclear chromosome; perinuclear region of cytoplasm; nuclear chromatin; chromatin; nucleus
Molecular Function: ATP-dependent DNA helicase activity; protein C-terminus binding; nuclease activity; DNA binding; hydrolase activity; manganese ion binding; endonuclease activity; exonuclease activity; 3'-5' exonuclease activity
Biological Process: negative regulation of virion penetration into host cell; sister chromatid cohesion; positive regulation of kinase activity; negative regulation of DNA endoreduplication; DNA repair; positive regulation of protein amino acid autophosphorylation; chromosome organization and biogenesis; DNA duplex unwinding; cell proliferation; meiotic cell cycle; nucleotide-excision repair; intra-S DNA damage checkpoint; base-excision repair; mitotic cell cycle G2/M transition DNA damage checkpoint; double-strand break repair; synapsis; telomere maintenance; response to DNA damage stimulus; DNA metabolic process
Research Articles on MRE11A
1. Impairment of Mre11 complex functions promotes the progression of mammary hyperplasias into invasive and metastatic breast cancers
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
