Ionized calcium-binding adapter molecule 1, Iba-1

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

Mouse IgG2b

Mouse

1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.

Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Small volumes of anti-AIF-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Product Description: Monoclonal antibody 1022-5 reacts with Allograft-inflammatory factor-1 (AIF-1) polypeptide, also known as Ionized calcium-binding adapter molecule 1, Iba-1. AIF-1 is a 17 kDa IFN-gamma inducible Ca2+-binding EF-hand protein, associated with chronic inflammatory processes. AIF-1 is constitutively expressed in lymphatic tissue and is up-regulated in monocytes/macrophages under various pathological conditions such as chronic rejection of allografts and in autoimmune lesions. In the brain, a subset of microglial cells constitutively express AIF-1. Increased numbers of AIF-1 immunoreactive macrophage/microglial cells were observed in focal brain infarctions, traumatic brain injury, gliomas, uveitis and in inflammatory lesions of a rat model of autoimmune disease, autoimmune encephalomyelitis and neuritis. Several Ca2+-binding peptides have been associated with monocyte activation, in particular members of the S-100 supergene-family. AIF-1 is a Ca2+-binding peptide which is not a member of the S-100 gene cluster but is located within the HLA class III genomic region. The monoclonal antibody 1022-5 is cross reactive with rat Allograft-inflammatory factor-1 (rat AIF-1). Human and rat amino acids sequences are 98% homologous.

Immunoassay (IA), P, Western Blot (WB)

Application Use: For immunohistology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For immunohistology on paraffin sections, a combination of microwave and sodium citrate solution is recommended for antigen retrieval.

14,748 Da

allograft inflammatory factor 1

allograft inflammatory factor 1; protein G1; interferon gamma responsive transcript; ionized calcium-binding adapter molecule 1; allograft inflammatory factor-1 splice variant Hara-1

Allograft inflammatory factor 1

G1; IBA1; AIF-1??[Similar Products]

AIF1_HUMAN

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

AIF1: Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T- lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: perinuclear region of cytoplasm; lamellipodium; cytoplasm; perikaryon; actin filament; cytosol; nucleus; phagocytic cup

Molecular Function: actin filament binding; calcium ion binding

Biological Process: actin filament bundle formation; positive regulation of nitric oxide biosynthetic process; negative regulation of smooth muscle cell proliferation; positive regulation of smooth muscle cell proliferation; Rac protein signal transduction; microglial cell activation; cellular response to hormone stimulus; actin filament polymerization; positive regulation of muscle hyperplasia; cellular response to extracellular stimulus; positive regulation of T cell proliferation; positive regulation of protein amino acid phosphorylation; response to axon injury; response to electrical stimulus; inflammatory response; phagocytosis, engulfment; negative regulation of apoptosis

1. Schluesener, H et al; Allograft-inflammatory factor-1 in rat experimental autoimmune encephalomyelitis, neuritis, and uveitis: expression by activated macrophages and microglial cells. Glia 1998, 24: 244 2. Beschorner, R et al; Differential regulation of the monocytic calcium-binding peptides macrophage-inhibiting factor related protein-8 (MRP8/S100A8) and allograft inflammatory factor-1 (AIF-1) following human traumatic brain injury. Acta neuropathol 2000, 100: 627 Deininger, M et al; The allograft inflammatory factor-1 in Creutzfeldt-Jakob disease brains. Neuropathol Appl Neurobiol 2003, 29: 389

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