Full Product Name
ADH4 siRNA (Human)
Product Synonym Names
Alcohol dehydrogenase 4; Alcohol dehydrogenase class II pi chain
Product Gene Name
ADH4 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P08319
Specificity
ADH4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ADH4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ADH4 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ADH4 sirna
siRNA to inhibit ADH4 expression using RNA interference
Applications Tested/Suitable for ADH4 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ADH4. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000661.2
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NCBI GenBank Nucleotide #
NM_000670.4
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UniProt Primary Accession #
P08319
[Other Products]
UniProt Secondary Accession #
Q8TCD7; A8K470; B4DIE7; C9J4A9[Other Products]
UniProt Related Accession #
P08319[Other Products]
Molecular Weight
42,607 Da
NCBI Official Full Name
alcohol dehydrogenase 4 isoform 2
NCBI Official Synonym Full Names
alcohol dehydrogenase 4 (class II), pi polypeptide
NCBI Official Symbol
ADH4??[Similar Products]
NCBI Official Synonym Symbols
ADH-2; HEL-S-4
??[Similar Products]
NCBI Protein Information
alcohol dehydrogenase 4
UniProt Protein Name
Alcohol dehydrogenase 4
UniProt Synonym Protein Names
Alcohol dehydrogenase class II pi chain
Protein Family
Alcohol dehydrogenase
UniProt Gene Name
ADH4??[Similar Products]
UniProt Entry Name
ADH4_HUMAN
NCBI Summary for ADH4
This gene encodes class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes. [provided by RefSeq, Jul 2008]
UniProt Comments for ADH4
ADH4: class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes. [provided by RefSeq, Jul 2008]
Protein type: EC 1.1.1.1; Amino Acid Metabolism - tyrosine; Xenobiotic Metabolism - metabolism by cytochrome P450; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Cofactor and Vitamin Metabolism - retinol; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Oxidoreductase; Lipid Metabolism - fatty acid
Chromosomal Location of Human Ortholog: 4q22
Cellular Component: cytosol
Molecular Function: zinc ion binding; NADPH:quinone reductase activity; alcohol dehydrogenase activity; all-trans retinal binding; ethanol binding; alcohol dehydrogenase activity, zinc-dependent; aldehyde reductase activity; benzaldehyde dehydrogenase activity; NAD binding; retinol binding; oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor; retinol dehydrogenase activity
Biological Process: retinol metabolic process; xenobiotic metabolic process; alcohol metabolic process; alcohol catabolic process; aldehyde metabolic process; retinoid metabolic process; ethanol oxidation
Research Articles on ADH4
1. This study confirmed the genetic heterogeneity of cluster headache, suggesting that mutations in the ADH4 gene might be related to cluster headache in a subset of cases.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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