Product Name
Cytochrome P450 24A1 (CYP24A1), Blocking Peptide
Full Product Name
Cytochrome P450 24A1 Blocking Peptide
Product Synonym Names
CYP24; 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 24-OHase; Vitamin D(3) 24-hydroxylase; Cytochrome P450 24A1; Cytochrome P450-CC24
Product Gene Name
CYP24A1 blocking peptide
[Similar Products]
Cytochrome P450 24A1 peptide (MBS8243549) is used for blocking the activity of Cytochrome P450 24A1 antibody (MBS8242991)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q07973
Species Reactivity
Human, Monkey
Form/Format
Lyophilized Powder
Quality Control
The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Other Notes
Small volumes of CYP24A1 blocking peptide vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CYP24A1 blocking peptide
The peptide is used to block Anti-Cytochrome P450 24A1 Antibody reactivity.
Applications Tested/Suitable for CYP24A1 blocking peptide
Blocking (BL)
Application Notes for CYP24A1 blocking peptide
Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4 degree C for overnight or at room temperature for 2 hours.
NCBI/Uniprot data below describe general gene information for CYP24A1. It may not necessarily be applicable to this product.
NCBI Accession #
Q07973.2
[Other Products]
UniProt Primary Accession #
Q07973
[Other Products]
UniProt Secondary Accession #
Q15807; Q32ML3; Q5I2W7[Other Products]
UniProt Related Accession #
Q07973[Other Products]
Molecular Weight
43,051 Da
NCBI Official Full Name
1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
NCBI Official Synonym Full Names
cytochrome P450 family 24 subfamily A member 1
NCBI Official Symbol
CYP24A1??[Similar Products]
NCBI Official Synonym Symbols
CP24; HCAI; CYP24; P450-CC24
??[Similar Products]
NCBI Protein Information
1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
UniProt Protein Name
1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
UniProt Synonym Protein Names
Cytochrome P450 24A1; Cytochrome P450-CC24
Protein Family
1,25-dihydroxyvitamin D(3) 24-hydroxylase
UniProt Gene Name
CYP24A1??[Similar Products]
UniProt Synonym Gene Names
CYP24; 24-OHase; Vitamin D(3) 24-hydroxylase??[Similar Products]
UniProt Entry Name
CP24A_HUMAN
NCBI Summary for CYP24A1
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for CYP24A1
CYP24A1: Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25- hydroxyvitamin D(3)) and calcitriol (1-alpha,25-dihydroxyvitamin D(3)). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23- hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D(3)-26,23-lactone as end product. Defects in CYP24A1 are the cause of hypercalcemia infantile (HCAI). HCAI is a disorder characterized by abnormally high level of calcium in the blood, failure to thrive, vomiting, dehydration, and nephrocalcinosis. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 1.14.13.126; Oxidoreductase; Mitochondrial
Chromosomal Location of Human Ortholog: 20q13
Cellular Component: mitochondrial inner membrane; mitochondrion; nucleoplasm; nucleus; plasma membrane
Molecular Function: 1-alpha,25-dihydroxyvitamin D3 (1,25-(OH)2D3) 24-hydroxylase activity; 25-hydroxycholecalciferol-24-hydroxylase activity; heme binding; iron ion binding; oxidoreductase activity
Biological Process: fat-soluble vitamin metabolic process; osteoblast differentiation; response to vitamin D; vitamin D catabolic process; vitamin D metabolic process; vitamin metabolic process; xenobiotic metabolic process
Disease: Hypercalcemia, Infantile
Research Articles on CYP24A1
1. Vitamin D-binding protein SNPs are associated with prostate cancer; low 25(OH)D metabolism score and CYP24A1 and CYP27B1 variants are associated with grade
Precautions
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Disclaimer
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